کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1990040 | 1540673 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The catalytic subunit of DNA-dependent protein kinase is downstream of ATM and feeds forward oxidative stress in the selenium-induced senescence response
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CM-H2DCFDAPBSMSEADNA-PKcsSA-β-galNACNHEJNa2SeO3DAPIMEFsDSBs4,6-diamidino-2-phenylindole - 4،6-دیامیدین-2-فنیلینولN-acetylcysteine - N-استیل سیستئینROS - ROSMethylseleninic acid - اسید متیل سلینینیکATM - خودپردازTEMPO - زمانsodium selenite - سلنیت سدیمdouble-strand breaks - شکست دو ردیفPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmouse embryonic fibroblasts - موش فیبروبلاست جنینیNonhomologous end-joining - پیوستن به انتهای غیرخطیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Selenium induces a senescence response in cells through induction of ataxia-telangiectasia mutated (ATM) and reactive oxygen species (ROS). Although a role of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) in DNA double-strand break repair is established, it is unclear how these proteins function in response to selenium-induced oxidative stress and senescence induction. In this study, we demonstrated that pretreating normal human diploid fibroblasts with DNA-PK kinase inhibitor NU 7026 suppressed selenium-induced senescence response. Selenium treatment induced phosphorylation of DNA-PKcs on Thr-2647 and Ser-2056, the extent of which was decreased in the presence of ATM kinase inhibitor KU 55933 or the antioxidants N-acetylcysteine or 2,2,6,6-tetramethylpiperidine-1-oxyl. In contrast, the selenium-induced phosphorylation of ATM on Ser-1981 was not affected by NU 7026. Cells deficient in DNA-PKcs or pretreated with NU 7026 or N-acetylcysteine were defective in selenite-induced ROS formation. Taken together, these results indicate a distinct role of DNA-PKcs, in which this kinase can respond to and feed forward selenium-induced ROS formation and is placed downstream of ATM in the resultant senescence response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 24, Issue 5, May 2013, Pages 781-787
Journal: The Journal of Nutritional Biochemistry - Volume 24, Issue 5, May 2013, Pages 781-787
نویسندگان
Caroline R.B. Rocourt, Min Wu, Benjamin P.C. Chen, Wen-Hsing Cheng,