کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2089471 | 1545791 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of murine monoclonal antibodies to methamphetamine and methamphetamine analogues
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کلمات کلیدی
DCCBOCRIATFAN-hydroxysuccinimidedicyclohexylcarbodiimideKLHmAbsTBSNHSSMCCTHFStereospecificityEDCIgGmAbdi-tert-butyl dicarbonatePBSHOBt1-hydroxybenzotriazole - 1-هیدروکسی بنزوتریازولBSA - BSAPd/C - Pd / Cbovine serum albumin - آلبومین سرم گاوMonoclonal antibody - آنتی بادی مونوکلونالTrifluoroacetic acid - اسید TrifluoroaceticEcstasy - اکستازی immunoglobulin G - ایمونوگلوبولین GTris-buffered saline - تریس بافر شورELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاtetanus toxoid - توکسوئین تانتانسCNS - دستگاه عصبی مرکزیradioimmunoassay - رادیوایمونواسیcentral nervous system - سیستم عصبی مرکزیMethamphetamine - متیل آمفتامینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریAffinity - وابستگیPalladium on carbon - پالادیوم در کربنoptical density - چگالی نوریkeyhole limpet hemocyanin - کلم هول limpet هموسیانین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of murine monoclonal antibodies to methamphetamine and methamphetamine analogues Development of murine monoclonal antibodies to methamphetamine and methamphetamine analogues](/preview/png/2089471.png)
چکیده انگلیسی
Methamphetamine and ecstasy are addictive drugs that cause major health problems in young people. Here we report on the development of high-affinity monoclonal antibodies to methamphetamine and its analogues, which may constitute powerful tools for antibody-based therapy. Six haptens, methamphetamine and ecstasy analogues, were synthesized, linked to a carrier protein and injected into mice. Several specific monoclonal antibodies were subsequently obtained following fusion of splenocytes from the immunized animals, with Sp2/O cells. Antibody specificity was fully investigated by competition ELISA, using a series of analogues, to identify specific amphetamine and/or ecstasy-specific antibodies. Antibody affinity was estimated to be in the range of 108 Mâ 1 with an enantiomeric hapten. Finally, two characteristic hybridoma clones (DAS-M243-6 H5 and DAS-M278-4 B12), secreting specific and potent mAbs were isolated. The development of drug-specific antibodies as in this study may provide promising therapeutic insight into how to neutralize methamphetamine in vivo during acute intoxication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 309, Issues 1â2, 20 February 2006, Pages 1-10
Journal: Journal of Immunological Methods - Volume 309, Issues 1â2, 20 February 2006, Pages 1-10
نویسندگان
Yannic Danger, Caroline Gadjou, Anne Devys, Hervé Galons, Dominique Blanchard, Gilles Folléa,