کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2579766 | 1561583 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bothriurus bonariensis scorpion venom activates voltage-dependent sodium channels in insect and mammalian nervous systems
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کلمات کلیدی
TTXtetrodotoxin citrateBBVNa+/Ca2+ exchangersNCXVGSCHBSinositol triphosphateIP34-APdays in vitroAMPA4-aminopyridine - 4-آمینوپیریدینHEPES-buffered saline - HEPES-Buffered salineα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid - اسید α-آمینو 3-هیدروکسی-5-متیل-4-ایزوکسول پپونیکArthropods - بندپایانCNS - دستگاه عصبی مرکزیDIV - دیوPoison - سمNeurotoxicity - سمیت عصبیcentral nervous system - سیستم عصبی مرکزیperipheral nervous system - سیستم عصبی پیرامونیNeuromuscular blockade - محرومیت عضلانی عضلانیCalcium influx - هجوم کلسیمPNS - کارمندان دولتvoltage-gated Ca2+ channels - کانال های Ca2 + دارای ولتاژvoltage-gated sodium channels - کانال های سدیم ولتاژGlu - گلوglutamate - گلوتامات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Animal venoms have been widely recognized as a major source of biologically active molecules. Bothriurus bonariensis, popularly known as black scorpion, is the arthropod responsible for the highest number of accidents involving scorpion sting in Southern Brazil. Here we reported the first attempt to investigate the neurobiology of B. bonariensis venom (BBV) in the insect and mammalian nervous system. BBV (32 μg/g) induced a slow neuromuscular blockade in the in vivo cockroach nerve-muscle preparations (70 ± 4%, n = 6, p < 0.001), provoking repetitive twitches and significantly decreasing the frequency of spontaneous leg action potentials (SNCAPs) from 82 ± 3 minâ1 to 36 ± 1.3 minâ1 (n = 6, p < 0.05), without affecting the amplitude. When tested in primary cultures of rat hippocampal cells, BBV induced a massive increase of Ca2+ influx (250 ± 1% peak increase, n = 3, p < 0.0001). The disturbance of calcium homeostasis induced by BBV on the mammalian central nervous system was not accompanied by cellular death and was prevented by the co-treatment of the hippocampal cells with tetrodotoxin, a selective sodium channel blocker. The results suggest that the biological activity of BBV is mostly related to a modulation of sodium channels function. Our biological activity survey suggests that BBV may have a promising insecticidal and therapeutic potential.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 258, 25 October 2016, Pages 1-9
Journal: Chemico-Biological Interactions - Volume 258, 25 October 2016, Pages 1-9
نویسندگان
Douglas Silva dos Santos, Evelise Leis Carvalho, Jeferson Camargo de Lima, Ricardo Vaz Breda, Raquel Soares Oliveira, Thiago Carrazoni de Freitas, Simone Denise Salamoni, Michelle Flores Domingues, Angela Regina Piovesan, Juliano Tomazzoni Boldo,