کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2582102 | 1130221 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cleavage of focal adhesion kinase is an early marker and modulator of oxidative stress-induced apoptosis
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کلمات کلیدی
TSAPTKNACHerbimycin AFAKN-acetyl-l-cysteineThimerosalHCEROS - ROSThiosalicylic acid - اسید تیستالسیلیکOxidative stress - تنش اکسیداتیوApoptosis - خزان یاختهایGenistein - ماده جنستئینProteolysis - پروتئولیزProtein tyrosine kinase - پروتئین تیروزین کینازcaspase - کسپاز یا کاسپازfocal adhesion kinase - کیناز چسبندگی کانونیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cleavage of focal adhesion kinase is an early marker and modulator of oxidative stress-induced apoptosis Cleavage of focal adhesion kinase is an early marker and modulator of oxidative stress-induced apoptosis](/preview/png/2582102.png)
چکیده انگلیسی
Focal adhesion kinase (FAK) is a signaling molecule associated with cell survival. Previously, we showed that thimerosal, a reactive oxygen species (ROS) generator, can acutely induce FAK tyrosine phosphorylation (within minutes) and chronically induce apoptosis (within days) by redox modulation in HeLa S cells. In the present study, we report that a prolonged oxidative stress by thimerosal induces a remarkable cleavage of FAK, which is accompanied with apoptosis. In fact, the kinetics of FAK cleavage has a good correlation with and actually preceding the apoptosis that was independent of anoikis. The effects were almost completely blocked by the pretreatment with either N-acetyl-l-cysteine (ROS scavenger) or Z-VAD-FMK (pan-caspase inhibitor), suggesting ROS-induced caspase activation as a key mechanism. They could be also reproduced by hydrogen peroxide alone, which appeared to be responsible for thimerosal-mediated oxidative stress-induced apoptosis. Additionally, the down regulation of FAK with antisense oligonucleotide dramatically augmented thimerosal-induced apoptosis. We could observe similar results using human corneal epithelial cells. Taken together, our results show that FAK is a critical cellular target of caspases during oxidative stress (particularly by hydrogen peroxide), resulting in the acceleration of subsequent apoptosis regardless of the anchorage status of cells. From the present results, it is more likely that not cell detachment but the proteolytic cleavage (or inhibition) of FAK is a key modulator as well as a promising indicator of apoptosis in epithelial cells under oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 171, Issue 1, 10 January 2008, Pages 57-66
Journal: Chemico-Biological Interactions - Volume 171, Issue 1, 10 January 2008, Pages 57-66
نویسندگان
Md. Firoz Mian, Changkeun Kang, Seunghwan Lee, Jang Hyun Choi, Sun Sik Bae, Sun-Hee Kim, Yun-Hee Kim, Sung Ho Ryu, Pann-Ghill Suh, Jong-Shu Kim, Euikyung Kim,