کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2603204 | 1133812 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytotoxicity of atorvastatin and simvastatin on primary rainbow trout (Oncorhynchus mykiss) hepatocytes
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کلمات کلیدی
PECOrganic anion transporting polypeptide 1B1OATP1B1HMGRGLMCyP3-hydroxy-3-methylglutaryl coenzyme A reductase - 3-هیدروکسی-3-متیل گلوتاریل کوآنزیم A ردوکتازHepatocytes - hepatocytesAtorvastatin - آتورواستاتینAlamar Blue - آلامار آبیMevalonic acid - اسید MevalonicCytotoxicity - سمیت سلولیcytochrome P-450 - سیتوکروم P-450Simvastatin - سیمواستاتینPredicted environmental concentration - غلظت محیط زیست پیش بینی شدهRainbow trout - قزل آلای رنگین کمانMVA - مالیات بر ارزش افزودهGLM, General Linear Model - مدل خطی عمومی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Statins are cholesterol-lowering pharmaceuticals and commonly prescribed drugs in European countries. Their discharge into the aquatic environment has increased in the last few years and they are present at detectable levels in most sewage effluents. The aim of the present study was to quantify the cytotoxic effects of acid and lactone forms of two statins, atorvastatin and simvastatin, as well as selected metabolites (ortho- and para-hydroxy atorvastatin acid, ortho-hydroxy atorvastatin lactone, simvastatin hydroxyl carboxylic acid, and 3â²â²hydroxy simvastatin lactone) to hepatocytes from rainbow trout (Oncorhynchus mykiss). Hepatocytes were exposed for 24, 48, and 72 h to different concentrations of each test substance (0.4-400 μM). Cytotoxicity was measured as metabolic inhibition and loss of membrane integrity with the fluorescent probes alamar blue (AB) and 5-carboxyfluorescein diacetate, acetoxymethyl ester (CFDA-AM), respectively. Atorvastatin, simvastatin, and ortho-hydroxy atorvastatin lactone had dose-dependent cytotoxic effects on hepatocytes. Simvastatin was more toxic than atorvastatin and the lactone form more toxic than the acid form. Exposure time affected atorvastatin and ortho-hydroxy atorvastatin lactone but not simvastatin toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 24, Issue 6, September 2010, Pages 1610-1618
Journal: Toxicology in Vitro - Volume 24, Issue 6, September 2010, Pages 1610-1618
نویسندگان
Kathrin Sabine Ellesat, Knut-Erik Tollefsen, Anders Ã
sberg, Kevin V. Thomas, Ketil Hylland,