کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3202158 | 1201967 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients](/preview/png/3202158.png)
BackgroundRecently the immune regulatory role of T cell–derived IL-10 in allergic disease has been extensively studied. In contrast, there is mounting evidence that IL-10 might also have a role in the perpetuation of allergic inflammation and fibrotic remodeling. It has been reported that alternatively (IL-4) activated macrophages (aaMΦ) produce large quantities of IL-10 and lack IL-12 production.ObjectiveBearing this in mind, we hypothesized whether functionally different properties of IL-10–producing monocytes could be identified.MethodsIntracellular cytokine expression of IL-10, IL-12, and IL-6 in peripheral blood CD14+ monocytes was measured in 19 atopic patients and 18 healthy control subjects by means of flow cytometry. In addition, IL-10–secreting monocytes were sorted by means of flow cytometry. Capabilities of these cells regarding further differentiation, accessory cell capacity, and surface molecule expression were analyzed.ResultsOur data show a dichotomous expression pattern of either IL-10 or IL-12p40/p70 in peripheral blood monocytes after LPS stimulation. Compared with healthy control subjects, the percentage of IL-10–producing monocytes was significantly increased in atopic patients. IL-10–secreting monocytes were isolated by using an IL-10 secretion assay, and functional analysis of these sorted cells revealed that IL-10–secreting monocytes preferentially differentiate into suppressor of cytokine signaling 3 expressing aaMΦ, which perpetuate TH2 immune response.ConclusionOur study shows the existence of an IL-10–producing monocyte subset, which is increased in atopic disease and which might facilitate allergic inflammation and fibrotic remodeling by differentiation into aaMΦ.Clinical implicationsControlling aaMΦ in TH2-driven inflammatory processes might be a novel target for intervention strategies.
Journal: Journal of Allergy and Clinical Immunology - Volume 119, Issue 2, February 2007, Pages 464–471