کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3415672 | 1593653 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intracellular bacteriolysis triggers a massive apoptotic cell death in Shigella-infected epithelial cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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![عکس صفحه اول مقاله: Intracellular bacteriolysis triggers a massive apoptotic cell death in Shigella-infected epithelial cells Intracellular bacteriolysis triggers a massive apoptotic cell death in Shigella-infected epithelial cells](/preview/png/3415672.png)
چکیده انگلیسی
Infected epithelial cells, which act as a first barrier against pathogens, seldom undergo apoptosis. Rather, infected epithelial cells undergo a slow cell death that displays hallmarks of necrosis. Here, we demonstrate that rapid intracellular lysis of Shigella flexneri, provoked by either the use of a diaminopimelic acid auxotroph mutant or treatment of infected cells with antibiotics of the β-lactam family, resulted in a massive and rapid induction of apoptotic cell death. This intracellular bacteriolysis-mediated apoptotic death (IBAD) was characterized by the specific involvement of the mitochondrial-dependent cytochrome c/Apaf-1 axis that resulted in the activation of caspases-3, -6 and -9. Importantly, Bcl-2 family members and the NF-κB pathway seemed to be critical modulators of IBAD. Finally, we identified that IBAD was also triggered by Salmonella enterica serovar Typhimurium but not by the Gram-positive bacteria, Listeria monocytogenes. Together, our results demonstrate that, contrary to previous findings, epithelial cells are intrinsically able to mount an efficient apoptotic cell death response following infection. Indeed, apoptosis in normal circumstances is masked by powerful anti-apoptotic mechanisms, which are overcome in IBAD. Our results also uncover an unexpected consequence of the treatment of infected cells with certain classes of antibiotics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 10, Issues 10â11, AugustâSeptember 2008, Pages 1114-1123
Journal: Microbes and Infection - Volume 10, Issues 10â11, AugustâSeptember 2008, Pages 1114-1123
نویسندگان
Ivan Tattoli, Luigi Lembo-Fazio, Giulia Nigro, Leticia A.M. Carneiro, Elisabetta Ferraro, Giacomo Rossi, Maria Celeste Martino, Maria Egle de Stefano, Francesco Cecconi, Stephen E. Girardin, Dana J. Philpott, Maria Lina Bernardini,