کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5452899 | 1513833 | 2017 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits
ترجمه فارسی عنوان
پلاسما جت فشار اتمسفر سرد، ویژگی های ویروالنسیتی کاندیدا آلبیکنس را تقویت می کند
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کلمات کلیدی
پلاسما، ضد قارچ، کاندیدا آلبیکنس، بیوفیلم ها، ویروسی شدن، سمیت مسمومیت،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
دانش مواد (عمومی)
چکیده انگلیسی
The occurrence of Candida-related infections depends on the interplay between fungal virulence factors and host's immune system. For this reason, interfering with fungal virulence is a promising approach to prevent or treat such diseases. The goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity and genotoxicity of He-CAPPJ to Vero cells. The effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Sub-inhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay and genotoxicity was investigated by micronucleus test. A promising effect of He-CAPPJ on morphogenesis was observed, with a reduction of almost 40 times in the filamentation rate compared to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. In conclusion, He-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity after short treatment times. Periods of exposure effective against biofilms were cytotoxic to Vero cells. He-CAPPJ did not show genotoxic effect. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Plasma Medicine - Volumes 7â8, December 2017, Pages 9-15
Journal: Clinical Plasma Medicine - Volumes 7â8, December 2017, Pages 9-15
نویسندگان
Aline Chiodi Borges, Thalita Mayumi Castaldelli Nishime, Konstantin Georgiev Kostov, Gabriela de Morais Gouvêa Lima, Aline Vidal Lacerda Gontijo, Juliana Nóbrega Martins Marchesotti de Carvalho, Roberto Yzumi Honda, Cristiane Yumi Koga-Ito,