Pharmacologic ascorbate induces neuroblastoma cell death by hydrogen peroxide mediated DNA damage and reduction in cancer cell glycolysis

Pharmacologic ascorbate forms H2O2, damages DNA and activates PARP. Activated PARP depletes NAD+, and therefore inhibited GAPDH activity, and ATP is depleted in neuroblastoma cells, leading to cell death. In the absence of PARP activation, fatal DNA damage accumulates and leads to cell death.82