کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510530 | 1539125 | 2017 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of water salinity on genes implicated in somatic growth, lipid metabolism and food intake in Pejerrey (Odontesthes bonariensis)
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کلمات کلیدی
EPAS15Δ6-desaturaseNUCB2/nesfatin-1HUFASFOLNAORFArachidonic acid - اسید آراشیدونیکdocosahexaenoic acid - اسید داکوزاگزوائونیکLinoleic acid - اسید لینولئیکLinolenic acid - اسید لینولینیکpoly unsaturated fatty acid - اسید چرب اشباع نشدهhighly unsaturated fatty acid - اسید چرب اشباع نشدهPUFA - اسید چرب چند غیراشباعrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدDHA - دوکوساهگزائنوئیک اسیدSunflower oil - روغن آفتابگردانFish oil - روغن ماهیWater salinity - شوری آبInsulin-like growth factor - فاکتور رشد مانند انسولینopen reading frame - قاب خواندن بازRace - مسابقهGrowth hormone - هورمون رشد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pejerrey, Odontesthes bonariensis, is an euryhaline fish of commercial importance in Argentina. This work aimed to determine if water salinity affects the expression of genes involved in somatic growth (gh; ghr-I; ghr-II; igf-I), lipid metabolism (Î6-desaturase) and food intake (nucb2/nesfatin-1). First, we identified the full-length cDNA sequences of Î6-desaturase (involved in lipid metabolism) and nesfatin-1 (an anorexigen). Then, pejerrey juveniles were reared during 8Â weeks in three different water salinity conditions: 2.5Â g/L (S2.5), 15Â g/L (S15) and 30Â g/L (S30) of NaCl. Brain, pituitary, liver and muscle samples were collected in order to analyze mRNA expression. The expression of gh and ghr-II mRNAs increased in the pituitary of fish reared at S2.5 and S30 compared with the S15 group. The expression of ghr-I was higher in the liver of S30 group compared to S2.5 and S15. Igf-I mRNA expression in liver increased with the increment of water salinity, while it decreased in the muscle of S15 and S30 groups. Î6-desaturase expression increased in S2.5 group compared to S15 in both liver and muscle. S30 caused a decrease in the Î6-desaturase expression in liver compared to S15. The S30 treatment produced an increase in nucb2/nesfatin-1 mRNA expression in the brain and liver compared to S2.5 and S15. The changes in gene expression observed could help pejerrey perform better during salinity challenges. The S30 condition would likely promote pejerrey somatic growth in the long term.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 210, August 2017, Pages 29-38
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 210, August 2017, Pages 29-38
نویسندگان
Juan Ignacio Bertucci, Mario Oswaldo Tovar, Ayelén Melisa Blanco, Pedro Gómez-Requeni, Suraj Unniappan, Luis Fabián Canosa,