کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5515575 | 1541907 | 2017 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative study of withanolide production and the related transcriptional responses of biosynthetic genes in fungi elicited cell suspension culture of Withania somnifera in shake flask and bioreactor
ترجمه فارسی عنوان
بررسی مقایسه ای تولید با آنولید و پاسخ های مربوط به رونویسی ژن های بیوسنتز در کشت سوسپانسیون سلولی آویشن در موش ها در فلاسک لرزش و بیوراکتور
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کلمات کلیدی
DXR1-deoxy-d-xylulose 5-phosphate reductoisomerase1-deoxy-D-xylulose 5-phosphate synthaseFPPSDXSCASMEPHMGRqPCR3-hydroxy-3-methylglutaryl coenzyme A reductase - 3-هیدروکسی-3-متیل گلوتاریل کوآنزیم A ردوکتازMurashige and Skoog - Murashige و Skoogsqualene synthase - اسکالن سنتازWithanolides - با آنولید هاBioreactor - بیورآکتور یا رآکتور زیستیfarnesyl pyrophosphate synthase - فارسیل پیرو فسفات سنتازMVA - مالیات بر ارزش افزودهMevalonate pathway - مسیر مولکولیquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیdry weight - وزن خشکhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراCell suspension cultures - کشت تعلیق سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Ashwagandha (Withania somnifera) is one of the most reputed medicinal plants in the traditional medicinal system. In this study, cell suspension culture of W. somnifera was elicited with cell homogenates of fungi (A. alternata, F. solani, V. dahliae and P. indica) in shake flask and the major withanolides like withanolide A, withaferin A and withanone were analysed. Simultaneously expression levels of key pathway genes from withanolides biosynthetic pathways were also checked via quantitative PCR in shake flask as well as in bioreactor. The results show that highest gene expression of 10.8, 5.8, 4.9, and 3.3 folds were observed with HMGR among all the expressed genes in cell suspension cultures with cell homogenates of 3% P. indica, 5% V. dahliae, 3% A. alternata and 3% F. solani, respectively, in comparison to the control in shake flask. Optimized concentration of cell homogenate of P. indica (3% v/v) was added to the growing culture in 5.0-l bioreactor under optimized up-scaling conditions and harvested after 22 days. The genes of MVA, MEP and withanolides biosynthetic pathways like HMGR, SS, SE, CAS, FPPS, DXR and DXS were up-regulated by 12.5, 4.9, 2.18, 4.65, 2.34, 1.89 and 1.4 folds, respectively in bioreactor. The enhancement of biomass (1.13 fold) and withanolides [withanolide A (1.7), withaferin A (1.5), and withanone (1.5) folds] in bioreactor in comparison to shake flask was also found to be in line with the up-regulation of genes of withanolide biosynthetic pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 114, May 2017, Pages 19-28
Journal: Plant Physiology and Biochemistry - Volume 114, May 2017, Pages 19-28
نویسندگان
Seema Ahlawat, Parul Saxena, Athar Ali, Shazia Khan, Malik Z. Abdin,