کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5540270 | 1553604 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and expression analysis of interferon-gamma in black seabream Acanthopagrus schlegelii
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کلمات کلیدی
ISGF3NLSGATA3NF-κBRdRpISGIFN-gammaJAK-STATISREIRFhour post infectionOCT1calcium/calmodulin kinase IITCID50Janus kinase-signal transducers and activators of transcriptionRGNNVGATA-binding protein 3IFN-stimulated gene factor 3PBSCaMKIIqRT-PCRhpiAP-1ORFPI3-K50% tissue culture infective dose - 50٪ کشت بافت آلودگیISGs - ISG هاRNA-dependent RNA polymerase - RNA پلیمراز وابسته به RNAinterferon - اینترفرونIFN - اینترفرون هاbase pair - جفت پایهBlack seabream - دریای سیاهnuclear localization signal - سیگنال محلی سازی هسته ایinterferon regulatory factor - عامل تنظیمی اینترفرونinterferon-stimulated response element - عنصر پاسخ تحریک شده توسط اینترفرونnuclear factor kB - فاکتور هسته ای kBopen reading frame - قاب خواندن بازphosphate buffer solution - محلول بافر فسفاتUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهquantitative real time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیactivator protein 1 - پروتئین فعال کننده 1mitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استPoly I:C - پلی I: Cpolyinosinic-polycytidylic acid - پلیسی سمی-پلیسییدیدیل اسیدInterferon-stimulated genes - ژن های تحریک کننده اینترفرونMAP kinase - کیناز MAPGas - گاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
Interferon gamma (IFN-γ) is a major component in immunological signaling and plays a key role in resisting viral infection. In this study, we identified and characterized an IFN-γ gene (AsIFN-γ) in the marine fish black seabream (Acanthopagrus schlegelii). We cloned AsIFN-γ genomic sequence, which comprises four exons, three introns and an upstream promoter including several conserved regulatory elements. The complete cDNA of AsIFN-γ was 816 bp in length and encoded a putative 194 amino acids (aa) protein with a 22 aa signal peptide, six α-helices and one nuclear localization signal (NLS). Multiple alignment showed that AsIFN-γ protein shared 31-60% identity with IFN-γ of other fish but low identity with fish IFN-γrel and IFN-γ of other vertebrates. AsIFN-γ was constitutively expressed in all examined tissues with the highest expression level in immune organs, such as spleen, gill and kidney. In black seabream infected by red spotted nervous necrosis virus (RGNNV), the expression of AsIFN-γ was significantly up-regulated in most tissues, and RGNNV infection in vitro also induced significant up-regulation of AsIFN-γ, indicating that AsIFN-γ was involved in immune response to RGNNV infection. Overexpression of AsIFN-γ in cultured Acanthopagrus schlegelii brain (AsB) cells rapidly and transiently stimulated the expression of JAK-STAT signaling pathway related genes including STAT1, STAT2 and IRF9, as well as the downstream antiviral genes MX1 and ISG15. Furthermore, overexpression of AsIFN-γ was able to significantly inhibit RGNNV replication and virus production in AsB cells. In summary, we identified a conserved IFN-γ gene of black seabream, and demonstrated the rapid and strong antiviral activities of AsIFN-γ against RGNNV in black seabream.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 70, November 2017, Pages 140-148
Journal: Fish & Shellfish Immunology - Volume 70, November 2017, Pages 140-148
نویسندگان
Yangxi Xiang, Wei Liu, Peng Jia, Yunlong Li, Yilin Jin, Limin Chen, Jing Zhang, Kuntong Jia, Meisheng Yi,