کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5562738 | 1562706 | 2017 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The plasticizer BBP selectively inhibits epigenetic regulator sirtuin during differentiation of C3H10T1/2 stem cell line
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کلمات کلیدی
NRF1HyperacetylationPGC1αSirtuinsTFAMFOXO1Nrf2Adipogenesis - آدیپوژنزBenzyl butyl phthalate - بنزیل بوتیل فتالاتBBP - تولید ناخالص داخلیMesenchymal stem cell - سلول های بنیادی مزانشیمیNuclear respiratory factor 1 - عامل تنفسی هسته ای 1nuclear respiratory factor 2 - عامل تنفسی هسته ای 2mitochondrial transcription factor A - عامل رونویسی میتوکندری Aendocrine disruptor - مختل کننده غدههای درون ریزperoxisome proliferator-activated receptor gamma coactivator 1 alpha - پراکسیسوم پرولاکتین گیرنده گاما گیرنده 1 آلفاForkhead box protein O1 - پروتئین جعبه ی جعبه ای O1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The plasticizer BBP selectively inhibits epigenetic regulator sirtuin during differentiation of C3H10T1/2 stem cell line The plasticizer BBP selectively inhibits epigenetic regulator sirtuin during differentiation of C3H10T1/2 stem cell line](/preview/png/5562738.png)
چکیده انگلیسی
Exposure to environmental chemicals can perturb an individual's metabolic set point, especially during critical periods of development, and as a result increase his or her propensity towards obesity that is manifested later in life and possibly in successive generations. We hypothesized that benzyl butyl phthalate (BBP), a widespread endocrine disruptor, may impair one important epigenetic regulator, sirtuin, in mesenchymal stem cells and induce adipogenesis. Our results showed that gene expression of two well-known adipogenic markers, aP2 and PPARγ, were significantly increased from day 2 to day 8 under 50 μM BBP exposure when compared to control in C3H10T1/2 stem cells (p < 0.05) and induced adipogenesis. Sirt1 gene expression was also significantly decreased at day 2, 4, 6, and 8 (p < 0.05). However, Sirt7 gene expression was decreased only at day 2 and 8 (p < 0.05) while other sirtuin transcriptional levels remained unaltered throughout. Furthermore, Sirt1 and Sirt3 protein expression was decreased (p < 0.05) and overall protein hyperacetylation was observed at day 8. Furthermore, FOXO1 and β-catenin, Sirt1 targets and adipogenesis regulators, were hyperacetylated at day 8. PGC1α, NRF1, NRF2, and Tfam, were also significantly decreased (p < 0.05). In conclusion, our study suggests for the first time that BBP, a potential epigenetic disruptor, can lead to increased adipogenesis and metabolic dysregulation by impairing vital epigenetic regulators.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 39, March 2017, Pages 75-83
Journal: Toxicology in Vitro - Volume 39, March 2017, Pages 75-83
نویسندگان
Jian Zhang, Mahua Choudhury,