کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5590083 | 1570095 | 2017 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evolution, expression analysis, and functional verification of Catharanthus roseus RLK1-like kinase (CrRLK1L) family proteins in pear (Pyrus bretchneideri)
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کلمات کلیدی
RLKsPerksASOCrRLK1Lmultiple em for motif elicitationHMMMEMEWGDAntisense oligonucleotides - oligonucleotide antisenseantisense oligonucleotide - oligonucleotide antisenseExpression pattern - الگوی بیانWhole-genome duplication - تقلید کامل ژنومMaximum likelihood - حداکثر احتمالCysteine-rich - غنی از سیتینEvolution - فرگشت Hidden Markov model - مدل پنهان مارکوف mya - میاmillion years ago - میلیون سال پیشSignal peptide - پپتید سیگنالneighbor-joining - پیوستن همسایهReceptor-like kinases - کیناز گیرنده مانندPear - گلابی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth. However, little is known about CrRLK1L in the wood of the pear fruit tree Pyrus bretchneideri. In this study, 26 CrRLK1L gene members were identified in pear and were grouped into six subfamilies according to phylogenetic analyses. Evolutionary analysis indicated that recent whole genome duplication (WGD) and dispersed gene duplications may contribute to the expansion of the CrRLK1L gene family in pear. Moreover, tissue-specific expression analyses suggested that CrRLK1Ls are involved in the development of various pear tissues. Subsequent qRT-PCR analyses indicated that CrRLK1Ls might play important roles in pollen tube growth. Finally, experiments with antisense oligonucleotides (ASO) demonstrated that PbrCrRLK1L26 have functions in pollen tube elongation and that PbrCrRLK1L3 regulates pollen tube rupture. These results will be useful for elaborating the biological roles of CrRLK1Ls in pear growth and development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 109, Issues 3â4, July 2017, Pages 290-301
Journal: Genomics - Volume 109, Issues 3â4, July 2017, Pages 290-301
نویسندگان
Kou Xiaobing, Qi Kaijie, Qiao Xin, Yin Hao, Liu Xing, Zhang Shaoling, Wu Juyou,