کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5649559 | 1407127 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MiR-142 Is Required for Staphylococcus aureus Clearance at Skin Wound Sites via Small GTPase-Mediated Regulation of the Neutrophil Actin Cytoskeleton
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CFUeGFPfMLPN-formylmethionyl-leucyl-phenylalanine3′-UTR - 3'-UTR3′-untranslated region - 3'-ناحیه ترجمه نشدهLYS - لیستMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAwild-type - نوع وحشیcolony-forming units - واحدهای تشکیل دهنده کلنیenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته است
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: MiR-142 Is Required for Staphylococcus aureus Clearance at Skin Wound Sites via Small GTPase-Mediated Regulation of the Neutrophil Actin Cytoskeleton MiR-142 Is Required for Staphylococcus aureus Clearance at Skin Wound Sites via Small GTPase-Mediated Regulation of the Neutrophil Actin Cytoskeleton](/preview/png/5649559.png)
چکیده انگلیسی
MicroRNAs (miRNAs) are small noncoding RNAs that regulate protein translation by binding to complementary target mRNAs. We previously identified two mature members of the miR-142 family, miR-142-5p and miR-142-3p, as inflammation-related miRNAs with potential roles in wound healing. Here, we demonstrated that these two miRNAs are prominently expressed in wound-infiltrated neutrophils and macrophages and play central roles in wound healing. We generated miR-142â/â mice using the exchangeable gene-trap method and showed that healing of Staphylococcus aureus-infected skin wounds was significantly delayed in miR-142â/â mice compared with that in wild-type mice. MiR-142â/â mice exhibited abnormal abscess formation at S. aureus-infected skin wound sites and were also more susceptible to horizontal transmission of wound infections. MiR-142â/â neutrophils showed altered phagocytosis as a consequence of chemotactic behavior, including enhanced F-actin assembly, disturbed cell polarity, and increased cell motility. We showed that these changes were linked to cytoskeletal regulation, and that expression of the small GTPases was markedly increased in miR-142â/â neutrophils. Collectively, our data demonstrate that the miR-142 family is indispensable for protection against S. aureus infection and its clearance at wound sites. MiR-142-3p and miR-142-5p play nonredundant roles in actin cytoskeleton regulation by controlling small GTPase translation in neutrophils at wound sites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 137, Issue 4, April 2017, Pages 931-940
Journal: Journal of Investigative Dermatology - Volume 137, Issue 4, April 2017, Pages 931-940
نویسندگان
Katsuya Tanaka, Sang Eun Kim, Hiroki Yano, Gaku Matsumoto, Ryoma Ohuchida, Yuhoko Ishikura, Masatake Araki, Kimi Araki, Seongjoon Park, Toshimitsu Komatsu, Hiroko Hayashi, Kazuya Ikematsu, Katsumi Tanaka, Akiyoshi Hirano, Paul Martin, Isao Shimokawa,