کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5766634 | 1628040 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synchronous overexpression of glutathione-S-transferase and cyanidase maintains the redox homeostasis âand improves cyanide remediation capacity in tobacco
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کلمات کلیدی
MDAPODCATTBAGSHGSTCDNB1-chloro-2,4-dinitrobenzene - 1-کلرو-2،4-دینیتروبنزنKCN - IP هاROS - ROSThiobarbituric acid - اسید تیوباربیتوریکCyanide - اسید سیاندریک و سیانورTobacco - توتون و تنباکوSOD - سدSynthetic biology - سنتتیک بیولوژیSuperoxide dismutase - سوکسوکس دیسموتازmalondialdehyde - مالون دی آلدهیدpotassium cyanide - پتاسیم سیانیدPeroxidase - پراکسیدازCatalase - کاتالازglutathione-S-transferase - گلوتاتیون S-ترانسفرازPhytoremediation - گیاه پالایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Synchronous overexpression of glutathione-S-transferase and cyanidase maintains the redox homeostasis âand improves cyanide remediation capacity in tobacco Synchronous overexpression of glutathione-S-transferase and cyanidase maintains the redox homeostasis âand improves cyanide remediation capacity in tobacco](/preview/png/5766634.png)
چکیده انگلیسی
Genetically engineered plants could provide feasible and environmentally safe approach for phytoremediation of CN compounds. However, the survival capacity of transgenic plants under high doses of CN is disappointing. One of the possible causes of such phytotoxicity is the CN-induced inhibition of antioxidant systems that lead to accumulation of reactive oxygen species (ROS). Therefore, simultaneous overexpression of a CN degrading enzyme along with an âoxidative stress-relieving protein could maintain the internal redox homeostasis and enhance CN-phytoremediation capacity under high doses of CN. In the present study, transgenic tobacco plants overexpressing the âbacterial cyanidase (CYND) and the cyanobacterial glutathione-S-transferase (GST) separately âand âin combination have been generated. Significant growth recovery was observed for all transgenic plants under 10Â mM CN compared to wild type plants. However, GSTÂ +Â CYND overexpressors showed the highest biomass accumulation under CN stress. Similarly, reduction in photosynthetic pigments and total carbohydrate levels were significantly recovered in transgenic plants especially at higher doses of CN (7.5 and 10.0Â mM). Moreover, both GST and GSTÂ +Â CYND transgenic plants showed elevated activities of SOD and CAT compared to the wild type upon exposure to CN. MDA content, as an indicator of lipid peroxidation, was significantly decreased in all transgenic plants. However, the lowest level of MDA was observed in GSTÂ +Â CYND transgenic lines. These results suggest that synchronous overexpression of GST with CYND genes improves CN remediation capacity of tobacco by improvement of the antioxidant scavenging systems and keeping ROS under control.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental and Experimental Botany - Volume 137, May 2017, Pages 220-227
Journal: Environmental and Experimental Botany - Volume 137, May 2017, Pages 220-227
نویسندگان
Rashad Kebeish, Yassin El-Ayouty, Ayman El-Naggar, Ahmed M. Saleh,