کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5839514 | 1123994 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease
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کلمات کلیدی
PC12autophagy-lysosomal pathway3MALC31-methyl-4-phenylpyridiniumNGFTH-irMPTP3-methyladenine - 3-متیل آدنینMPP+ - MPP +SNpc - SNPCALP - آلکالن فسفاتازAutophagy - اتوفاژیStriatum - استریاتومSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتParkinson's disease - بیماری پارکینسونELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاsubstantia nigra pars compacta - توده سیاه پارس متراکمMYH - خیلی ممنونDopamine - دوپامینlight chain 3 - زنجیره سبک 3Rat pheochromocytoma cells - سلولهای فئوکروموسیتوما موش صحراییUbiquitin-proteasome system - سیستم Ubiquitin-proteasomenerve growth factor - فاکتور رشد عصبLactacystin - لاکتاسیستینNeuroprotection - محافظت نورونی یا محافظت از عصبUPS - یو پی اس
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aim of studyModified Yeoldahanso-tang (MYH) is a Korean herbal formula, containing 10 herbs: Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd.), Acorus gramineus Soland. and Dimocarpus longan Lour. The constitutive ratio of the ten herbs is at 6:4:2:1:2:2:2:4:6:6 in dry weight. MYH has been used to treat amnesia, hypochondria and dementia in Korea. In this study, we explored the possibility of using MYH in the prevention and treatment of Parkinson's disease (PD). Specifically, we made an effort to demonstrate the neuroprotective effects of MYH using experimental methods similar to those used in a recent study of PD.Materials and methods1-Methyl-4-phenylpyridinium (MPP+) (400 μM) was used to induce cytotoxicity in NGF (nerve growth factor)-differentiated PC12 cells. Cell viability was measured using a MTT assay. Induction of autophagy by MYH in NGF-differentiated PC12 cells was measured using an immunoblotting assay with LC3 and beclin 1 antibodies. The proteasomal inhibitor lactacystin (10 μM) was used to cause UPS dysfunction in NGF-differentiated PC12 cells. Clearance of aggregated proteins by MYH was measured using an immunoblotting assay with an ubiquitin antibody. 1-Methyl-4-phenyl-1,2,3,6-tetrahydrophenylpyridine (MPTP) (20 mg/kg, 4 times i.p.) caused substantia nigra injuries in C57BL/6 mice. Dopamine (DA) neurons were identified using a tyrosine hydroxylase-immunohistochemistry (TH-IHC) assay with a rabbit anti-TH antibody.ResultsOur findings indicate that MYH provides protection against MPP+-induced injury in NGF-differentiated PC12 cell. And MYH provides neuroprotection against lactacystin-induced NGF-differentiated PC12 cell death, which effect is partially mediated by autophagy enhancement through enhanced degradation of aggregated proteins. Additionally, in a C57BL/6 mice model with MPTP-induced substantia nigra injuries, MYH inhibits both the loss of TH-positive neurons in the substantia nigra pars compacta (SNpc) and the reduction of the optical density of TH-IR fibers in the striatum (ST).ConclusionsAll of our results indicate that MYH treatment has neuroprotective effects that are partially mediated by autophagy enhancement. MYH may be a promising herbal formula for the prevention and treatment of neurodegenerative diseases, especially PD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 134, Issue 2, 24 March 2011, Pages 313-322
Journal: Journal of Ethnopharmacology - Volume 134, Issue 2, 24 March 2011, Pages 313-322
نویسندگان
Nayoung Bae, Taekwon Ahn, Sungkwon Chung, Myung Sook Oh, Hyeonseok Ko, Hyungun Oh, Gunhyuk Park, Hyun Ok Yang,