کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861021 | 1562712 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment of cosmetic ingredients in the in vitro reconstructed human epidermis test method EpiSkin⢠using HPLC/UPLC-spectrophotometry in the MTT-reduction assay
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کلمات کلیدی
RhCEDG SANCOCAS RNEURL ECVAMEITWLRchemical abstract service registry numberSCCsRHTMSDsOECDreconstructed human cornea-like epitheliumFDASOPRHEwithin laboratory reproducibilityR&IUN GHS - GHSMTT - MTTEuropean Union Reference Laboratory for Alternatives to Animal Testing - آزمایشگاه مرجع اتحادیه اروپا برای جایگزینی برای تست حیواناتEye irritation test - آزمون تحریک چشمstandard deviation - انحراف معیارReconstructed human epidermis - اپی ادیس انسانی بازسازی شدهSkin irritation - تحریک پوستPercentage - درصدtest guideline - دستورالعمل آزمونstandard operating procedure - روش های عملیاتی استانداردFood and Drug Administration - سازمان غذا و داروUnited Nations Globally Harmonized System of Classification and Labelling of Chemicals - سازمان ملل متحد سیستم جهانی طبقه بندی و برچسب زدن مواد شیمیاییOrganisation for Economic Co-operation and Development - سازمان همکاری اقتصادی و توسعهcoefficient of determination - ضریب تعیینCosmetic ingredients - مواد آرایشیMaterial safety data sheet - ورق ایمنی موادoptical density - چگالی نوریScientific Committee on Consumer Safety - کمیته علمی ایمنی مصرف کننده
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Assessment of cosmetic ingredients in the in vitro reconstructed human epidermis test method EpiSkin⢠using HPLC/UPLC-spectrophotometry in the MTT-reduction assay Assessment of cosmetic ingredients in the in vitro reconstructed human epidermis test method EpiSkin⢠using HPLC/UPLC-spectrophotometry in the MTT-reduction assay](/preview/png/5861021.png)
چکیده انگلیسی
Cosmetics Europe recently established HPLC/UPLC-spectrophotometry as a suitable alternative endpoint detection system for measurement of formazan in the MTT-reduction assay of reconstructed human tissue test methods irrespective of the test system involved. This addressed a known limitation for such test methods that use optical density for measurement of formazan and may be incompatible for evaluation of strong MTT reducer and/or coloured chemicals. To build on the original project, Cosmetics Europe has undertaken a second study that focuses on evaluation of chemicals with functionalities relevant to cosmetic products. Such chemicals were primarily identified from the Scientific Committee on Consumer Safety (SCCS) 2010 memorandum (addendum) on the in vitro test EpiSkin⢠for skin irritation testing. Fifty test items were evaluated in which both standard photometry and HPLC/UPLC-spectrophotometry were used for endpoint detection. The results obtained in this study: 1) provide further support for Within Laboratory Reproducibility of HPLC-UPLC-spectrophotometry for measurement of formazan; 2) demonstrate, through use a case study with Basazol C Blue pr. 8056, that HPLC/UPLC-spectrophotometry enables determination of an in vitro classification even when this is not possible using standard photometry and 3) addresses the question raised by SCCS in their 2010 memorandum (addendum) to consider an endpoint detection system not involving optical density quantification in in vitro reconstructed human epidermis skin irritation test methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 33, June 2016, Pages 105-117
Journal: Toxicology in Vitro - Volume 33, June 2016, Pages 105-117
نویسندگان
N. Alépée, J. Hibatallah, M. Klaric, K.R. Mewes, U. Pfannenbecker, P. McNamee,