کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6017845 | 1580179 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Altered miRNA expression is associated with neuronal fate in G93A-SOD1 ependymal stem progenitor cells
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کلمات کلیدی
SOD1SRY (sex determining region Y)-Box 2Cyclin D2JAG1SALSJagged 1SRY (sex determining region Y)-box 9CCND2SOCS1Sox2STAT3DLX2N-methyl-d-aspartateSox9NMDAglutamic acid decarboxylase 67GFAPOct-4 - 4 اکتبرfamilial ALS - ALS خانوادگیsporadic ALS - ALS پراکندهamyotrophic lateral sclerosis - اسکلروز جانبی آمیوتروفیکALS - بیماری اسکلروز جانبی آمیوتروفیکfALS - جعل اسنادsuppressor of cytokine signaling 1 - سرکوب کننده سیگنالینگ سیتوکین 1superoxide dismutase 1 - سوپر اکسید دیسموتاز 1phosphatase and tensin homolog - فسفاتاز و تنسین همولوگhairy and enhancer of split 1 - مودار و تقویت کننده تقسیم 1MicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAHes1 - هس 1Glial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالPten - ژن PTEN
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aims of the study were to demonstrate that epSPCs isolated from the spinal cord of G93A-SOD1 mice express neurogenic potential in vitro, and thus gain a better understanding of epSPC neural differentiation properties. For this purpose, we compared the ability of epSPCs from asymptomatic and symptomatic G93A-SOD1 and WT SOD1 transgenic mice to proliferate and differentiate into neural cells. Compared to control cells, G93A-SOD1 epSPCs differentiated more into neurons than into astrocytes, whereas oligodendrocyte proportions were similar in the two populations. G93A-SOD1 neurons were small and astrocytes had an activated phenotype. Evaluation of microRNAs, specific for neural cell fate and cell-cycle regulation, in G93A-SOD1 epSPCs showed that miR-9, miR-124a, miR-19a and miR-19b were differentially expressed. Expression analysis of the predicted miRNA targets allowed identification of a functional network in which Hes1, Pten, Socs1, and Stat3 genes were important for controlling epSPC fate. Our findings demonstrate that G93A-SOD1 epSPCs are a source of multipotent cells that have neurogenic potential in vitro, and might be a useful tool to investigate the mechanisms of neural differentiation in relation to miRNA expression whose modulation might constitute new targeted therapeutic approaches to ALS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Neurology - Volume 253, March 2014, Pages 91-101
Journal: Experimental Neurology - Volume 253, March 2014, Pages 91-101
نویسندگان
Stefania Marcuzzo, Dimos Kapetis, Renato Mantegazza, Fulvio Baggi, Silvia Bonanno, Claudia Barzago, Paola Cavalcante, Nicole Kerlero de Rosbo, Pia Bernasconi,