کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6074875 | 1203489 | 2016 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rab11a Is Essential for Lamellar Body Biogenesis in the Human Epidermis
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کلمات کلیدی
GTPCTRLRHEshRNATGNLROGTPaseTEWLshort hairpin RNA - RNA موی سر کوتاهLysosome-related organelle - ارگانل مربوط به لیسوزومTransepidermal water loss - از دست دادن آب دریچهReconstructed human epidermis - اپی ادیس انسانی بازسازی شدهCer - سرceramide - سرامیدTrans-Golgi network - شبکه Trans-Goljilamellar body - لامالار بدنControl - کنترلGuanosine triphosphate - گوانوزین تری فسفات
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Most of the skin barrier function is attributable to the outermost layer of the epidermis, the stratum corneum, which is composed of flattened, anucleated cells called corneocytes surrounded by a lipid-enriched lamellar matrix. The composition of the stratum corneum is directly dependent on the underlying granular keratinocytes, which are the last living cells in the stratified epidermis. Many components present in the intercorneocyte matrix are delivered by the underlying granular keratinocytes through a secretion process dependent on lysosome-related organelles called lamellar bodies. Because of the importance of lamellar bodies in the maintenance of the epidermal barrier, the mechanisms regulating their biogenesis must be better understood. In this study, we show that the Rab11a GTPase is highly expressed in terminally differentiated keratinocytes, where it is partly associated with lamellar bodies. Rab11a silencing in three-dimensional in vitro reconstructed human epidermis induces a barrier defect, a decrease in the amount of lipid found in the stratum corneum, a reduction in lamellar body density and secretion areas in granular keratinocytes, and the mis-sorting of lamellar body cargoes being driven to the lysosomal degradation pathway. Our results highlight the importance of Rab11a-dependent regulation of lamellar body biogenesis in keratinocytes and consequently on epidermal barrier homeostasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 136, Issue 6, June 2016, Pages 1199-1209
Journal: Journal of Investigative Dermatology - Volume 136, Issue 6, June 2016, Pages 1199-1209
نویسندگان
Marie Reynier, Sophie Allart, Elise Gaspard, Alain Moga, Dominique Goudounèche, Guy Serre, Michel Simon, Corinne Leprince,