کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6277108 | 1295748 | 2010 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular prion protein overexpression disturbs cellular homeostasis in SH-SY5Y neuroblastoma cells but does not alter p53 expression: a proteomic study
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کلمات کلیدی
PQBP1MTDCTransgelin 2GRB2PRNPsCJDPrPcGSTO1ANXA5TCTPFCS2-DEDMEMDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoP/S - P / SAnnexin V - آنکسیان Vtwo dimensional gel electrophoresis - الکتروفورز ژل دو بعدیsporadic Creutzfeldt–Jakob disease - بیماری کرایتزفلد-جاکوب پراکندهProtein folding - تاشدگی پروتئینTransgelin - ترانژلینfetal calf serum - سرم گوساله جنینSpermine synthase - سنتاز اسپرمfumarate hydratase - فومارات هیدراتازSignal transduction - هدایت سیگنالPpid - پاپGrowth factor receptor bound protein 2 - پروتئین متصل گیرنده عامل رشد 2Prion protein - پروتئین پریونcellular prion protein - پروتون پریون سلولیPenicillin/streptomycin - پنیسیلین / استرپتومایسینCytoskeleton - چارچوب یاخته، سیتواسکلتون، اسکلت سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The definite physiological role of the cellular prion protein (PrPc) remains elusive. There is ample in vitro and in vivo evidence suggesting a neuroprotective role for PrPc. On the other hand, several in vitro and in vivo studies demonstrated detrimental effects of PrPc overexpression through activation of a p53 pathway. Recently, we reported that transient overexpression of PrPc in human embryonic kidney 293 cells elicits proteome expression changes which point to deregulation of proteins involved in energy metabolism and cellular homeostasis. Here we report proteome expression changes following stable PrPc overexpression in human neuronal SH-SY5Y cells. In total 18 proteins that are involved in diverse biological processes were identified as differentially regulated. The majority of these proteins is involved in cell signaling, cytoskeletal organization and protein folding. Annexin V exhibited a several fold up-regulation following stable PrPc overexpression in SH-SY5Y cells. This finding has been reproduced in alternative, mouse N2a and human SK-N-LO neuroblastoma cell lines transiently overexpressing PrPc. Annexin V plays an important role in maintenance of calcium homeostasis which when disturbed can activate a p53-dependent cell death. Although we did not detect changes in p53 expression between PrPc overexpressing SH-SY5Y and control cells, deregulation of several proteins including annexin V, polyglutamine tract-binding protein-1, spermine synthase and transgelin 2 indicates disrupted cellular equilibrium. We conclude that stable PrPc overexpression in SH-SY5Y cells is sufficient to perturb cellular balance but insufficient to affect p53 expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 169, Issue 4, 15 September 2010, Pages 1640-1650
Journal: Neuroscience - Volume 169, Issue 4, 15 September 2010, Pages 1640-1650
نویسندگان
E. Weiss, S. Ramljak, A.R. Asif, B. Ciesielczyk, M. Schmitz, J. Gawinecka, W. Schulz-Schaeffer, C. Behrens, I. Zerr,