کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6391314 | 1628415 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preservation of viability and anti-Listeria activity of lactic acid bacteria, Lactococcus lactis and Lactobacillus paracasei, entrapped in gelling matrices of alginate or alginate/caseinate
ترجمه فارسی عنوان
حفاظت از حیات و فعالیت ضد لیستریاسیون باکتری های اسید لاکتیک، لکتوکوکوس لاکتیس و لاکتوباسیلوس پاراسازئی، در ماتریس های ژلینگ آلژینات یا آلژینات / کازئینات
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
In order to control undesirable microorganisms growth in foods, the performance of alginate and alginate-caseinate (an aqueous two-phase system) matrices entrapping lactic acid bacteria (LAB) (Lactobacillus paracasei LAB1 and Lactococcus lactis LAB3) was investigated. Polymeric matrices were initially loaded with LAB cells at â¼108â10 or â¼104â6 CFU mLâ1, and were monitored, in liquid and gelled form (beads), for 12 days at 30 °C. In the liquid form, maximum cell density (â¼109 CFU mLâ1) was reached after 24 h whatever the matrix. Then, the LAB population decreased but remained higher in alginate-caseinate matrices: 107 and 106 CFU mLâ1 of LAB3 cells were enumerated after 12 days in alginate-caseinate and in alginate matrices, respectively. Anti-Listeria activity (assayed by agar well diffusion method) did not vary much over 12 days and was also higher for cells entrapped in alginate-caseinate matrices. When matrices were gelled, similar trends were observed: at “Day 12”, LAB3 population was 104â5 and 102â3 CFU/bead, and, LAB1 population was 105â6 and 103â4 CFU/bead, in alginate-caseinate and alginate beads, respectively. Antimicrobial activity of alginate-caseinate beads containing LAB1 cells was quite constant over 12 days. The anti-Listeria activity of LAB cell-free supernatants incorporated in matrices with caseinate was also higher. In fact, the presence of caseinate was shown to promote both the survival of LAB cells and the release of their antimicrobial metabolites. Observation of liquid and gelled matrices by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) revealed a preferential localization of LAB cells in casein-rich microdomains which could affect favorably the efficiency of bipolymeric matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 47, January 2015, Pages 7-19
Journal: Food Control - Volume 47, January 2015, Pages 7-19
نویسندگان
Lucie Léonard, Olfa Beji, Christine Arnould, Elodie Noirot, Aline Bonnotte, Adem Gharsallaoui, Pascal Degraeve, Jeannine Lherminier, Rémi Saurel, Nadia Oulahal,