کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6494509 | 44808 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
nOHGGOHFOHFPPGGPPDCWCRTIDMAPPGPPIPPIsoprenoid - ایسپرونیدisopentenyl diphosphate - ایسپنتنیل دی فسفاتTerpene synthase - ترپن سنتازGeraniol - جریمهولdimethylallyl diphosphate - دی متیللید دی فسفاتfarnesyl diphosphate - فارسیل دی فسفاتFarnesol - فارنزولLinalool - لاینالولLOH - لوهانYeast - مخمرProtein engineering - مهندسی پروتئینNerolidol - نریدیلولdry cell weight - وزن سلول خشکGeranyl diphosphate - ژانایل دی فسفاتgeranylgeraniol - ژرانیلگرانیولgeranylgeranyl diphosphate - گوارانیلگرانیل دی فسفات
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Terpenes have numerous applications, ranging from pharmaceuticals to fragrances and biofuels. With increasing interest in producing terpenes sustainably and economically, there has been significant progress in recent years in developing methods for their production in microorganisms. In Saccharomyces cerevisiae, production of the 20-carbon diterpenes has so far proven to be significantly less efficient than production of their 15-carbon sesquiterpene counterparts. In this report, we identify the modular structure of geranylgeranyl diphosphate synthesis in yeast to be a major limitation in diterpene yields, and we engineer the yeast farnesyl diphosphate synthase Erg20p to produce geranylgeranyl diphosphate. Using a combination of protein and genetic engineering, we achieve significant improvements in the production of sclareol and several other isoprenoids, including cis-abienol, abietadiene and β-carotene. We also report the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed here can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 27, January 2015, Pages 65-75
Journal: Metabolic Engineering - Volume 27, January 2015, Pages 65-75
نویسندگان
Codruta Ignea, Fotini A. Trikka, Alexandros K. Nikolaidis, Panagiota Georgantea, Efstathia Ioannou, Sofia Loupassaki, Panagiotis Kefalas, Angelos K. Kanellis, Vassilios Roussis, Antonios M. Makris, Sotirios C. Kampranis,