کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7585458 | 1492038 | 2018 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Secondary structure changes induced by pulsed electric field affect antioxidant activity of pentapeptides from pine nut (Pinus koraiensis) protein
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کلمات کلیدی
MIRPEFnuclear magnetic resonance - رزونانس مغناطیسی هستهایNMR - تشدید مغناطیسی هستهای EPR - تشدید پارامغناطیس الکترونElectron paramagnetic resonance - تشدید پارامغناطیس الکترونcircular dichroism - رنگ تابی دورانیStructures - سازه هایAntioxidant activity - فعالیت آنتیاکسیدانیMid-infrared - مادون قرمزPulsed electric field - میدان الکتریکی پالسیPulsed electric field (PEF) - میدان الکتریکی پالسی (PEF)Pentapeptides - پنتاپپتیایدhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Secondary structure changes induced by pulsed electric field affect antioxidant activity of pentapeptides from pine nut (Pinus koraiensis) protein Secondary structure changes induced by pulsed electric field affect antioxidant activity of pentapeptides from pine nut (Pinus koraiensis) protein](/preview/png/7585458.png)
چکیده انگلیسی
We used a pulsed electric field (PEF) to treat four pentapeptides with similar amino acid sequences (KCHKP, KCHQP, QCHKP, and QCHQP). We then evaluated antioxidant activity of the pentapeptides according to ferric reducing antioxidant power (FRAP) and hydroxyl radical scavenging capacity. Structures of the peptides were determined by high performance liquid chromatography (HPLC), mid-infrared (MIR), circular dichroism (CD) spectroscopy, and nuclear magnetic resonance (NMR). The results indicated that antioxidant activities of KCHQP and QCHKP were increased by PEF, whereas those of KCHKP and QCHQP were reduced. The basic structures and functional groups of peptides were unaffected. PEF treatment reduced the α-helix contents of KCHQP and QCHKP, but increased those of KCHKP and QCHQP. Moreover, the chemical shifts at 14.46â¯ppm, 8.22â¯ppm, 7.87â¯ppm, 7.24â¯ppm, and 6.13â¯ppm attributable to hydrogen atoms of QCHKP shifted to the right, but the active hydrogens of QCHQP were not affected by PEF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 254, 15 July 2018, Pages 170-184
Journal: Food Chemistry - Volume 254, 15 July 2018, Pages 170-184
نویسندگان
Rong Liang, Sheng Cheng, Xiuying Wang,