کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7626478 | 1494580 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of choline kinase drives aberrant choline metabolism in esophageal squamous cell carcinomas
ترجمه فارسی عنوان
فعال سازی کولین کیناز باعث سوخت و ساز ناخواسته کولین در کارسینوم سلول سنگفرشی مری است
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کلمات کلیدی
DABTMAsESIESCCcPLA2PLS-DALPCFFAHCA3,3′-diaminobenzidine - 3،3'-diaminobenzidinePCA - PCAIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیHierarchical cluster analysis - تجزیه و تحلیل خوشه سلسله مراتبیPrincipal component analysis - تحلیل مولفههای اصلی یا PCAPhosphatidylcholines - فسفاتیدیل شولینphosphatidylethanolamine - فسفاتیدیلتانولامینcytosolic phospholipase A2 - فسفولیپاز A2 سیتوزولLysophosphatidylcholine - لیزوفسفاتیدیل کولینMetabolomics - متابولومیکtissue microarrays - میکروارگانیسم های بافتEsophageal squamous cell carcinoma - کارسینوم سلول سنگفرشی مریquality control - کنترل کیفیتCholine - کولینcholine kinase - کولین کینازelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Esophageal squamous cell carcinoma (ESCC) is a major health threat worldwide. Research focused on molecular events associated with ESCC carcinogenesis for diagnosis, treatment and prevention is needed. Our goal is to discover novel biomarkers and investigate the underlying molecular mechanisms of ESCC progression by employing a global metabolomic approach. Sera from 34 ESCC patients and 32 age and sex matched healthy controls were profiled using two-dimensional liquid chromatography-mass spectrometry (2D LC-MS). We identified 120 differential metabolites in ESCC patient serums compared to healthy controls. Several amino acids, serine, arginine, lysine and histidine were significantly changed in ESCC patients. Most importantly, we found dysregulated lipid metabolism as an important characteristic in ESCC patients. Several free fat acids (FFA) and carnitines were found down-regulated in ESCC patients. Choline was significantly increased and phosphatidylcholines (PC) were significantly decreased in ESCC serum. The high expression of choline and low expression of total PC in patient serum were associated with the high expression of choline kinase (Chok) and activated Kennedy pathway in ESCC cells. Chok expression can serve as a significant biomarker for ESCC prognosis. In conclusion, metabolite profiles in the ESCC patient serum were significantly different from those in the healthy controls. Phosphatidylcholines and Chok, the key enzyme in the PC metabolism pathway, may serve as novel biomarkers for ESCC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 155, 5 June 2018, Pages 148-156
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 155, 5 June 2018, Pages 148-156
نویسندگان
Wang Ma, Shuangyuan Wang, Tengfei Zhang, Erik Y. Zhang, Lina Zhou, Chunxiu Hu, Jane J. Yu, Guowang Xu,