کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8302063 | 1537718 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Docosahexaenoic acid biosynthesis via fatty acyl elongase and Î4-desaturase and its modulation by dietary lipid level and fatty acid composition in a marine vertebrate
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کلمات کلیدی
Dietary lipid levelLC-PUFAsEPADPAPUFAsALAα-linolenic acid - اسید α-لینولنیکArachidonic acid - اسید آراشیدونیکEicosapentaenoic acid - اسید ایکوزاپنتانوئیکdocosapentaenoic acid - اسید داکوزاپنتانوئیکdocosahexaenoic acid - اسید داکوزاگزوائونیکLinoleic acid - اسید لینولئیکFatty acid - اسید چربPolyunsaturated fatty acids - اسید چرب اشباع نشدهLong-chain polyunsaturated fatty acids - اسیدهای چرب اشباع نشده چای زنجیره ایFatty acid composition - ترکیب اسید چربARA - در حال حاضرDHA - دوکوساهگزائنوئیک اسیدVegetable oil - روغن سبزیجاتFish oil - روغن ماهیfatty acid methyl esters - متیل استرهای اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربLoA - مجازاتNutritional regulation - مقررات تغذیه ایFish meal - وعده ماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study presents the first “in vivo” evidence of enzymatic activity and nutritional regulation of a Î4-desaturase-dependent DHA synthesis pathway in the teleost Solea senegalensis. Juvenile fish were fed diets containing 2 lipid levels (8 and 18%, LL and HL) with either 100% fish oil (FO) or 75% of the FO replaced by vegetable oils (VOs). Fatty acyl elongation (Elovl5) and desaturation (Î4Fad) activities were measured in isolated enterocytes and hepatocytes incubated with radiolabeled α-linolenic acid (ALA; 18:3n â 3) and eicosapentaenoic acid (EPA; 20:5n â 3). Tissue distributions of elovl5 and Î4fad transcripts were also determined, and the transcriptional regulation of these genes in liver and intestine was assessed at fasting and postprandially. DHA biosynthesis from EPA occurred in both cell types, although Elovl5 and Î4Fad activities tended to be higher in hepatocytes. In contrast, no Î6Fad activity was detected on 14C-ALA, which was only elongated to 20:3n â 3. Enzymatic activities and gene transcription were modulated by dietary lipid level (LL > HL) and fatty acid (FA) composition (VO > FO), more significantly in the liver than in the intestine, which was reflected in tissue FA compositions. Dietary VO induced a significant up-regulation of Î4fad transcripts in the liver 6 h after feeding, whereas in fasting conditions the effect of lipid level possibly prevailed over or interacted with FA composition in regulating the expression of elovl5 and Î4fad, which were down-regulated in the liver of fish fed the HL diets. Results indicated functionality and biological relevance of the Î4 LC-PUFA biosynthesis pathway in S. senegalensis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 5, May 2015, Pages 588-597
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 5, May 2015, Pages 588-597
نویسندگان
Sofia Morais, Gabriel Mourente, Almudena MartÃnez, Noélia Gras, Douglas R. Tocher,