کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323009 | 1539889 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stearoyl-CoA desaturase regulates inflammatory gene expression by changing DNA methylation level in 3T3 adipocytes
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کلمات کلیدی
SFATyk2INHASCD1TGFB1CX3CL1GATA3IL6CXCL12MCP1ATF2FADDMUFAchemokine (C-X-C motif) ligand 12TNFαLPSinterleukin 6 signal transducerFASSAMIL6STLTBchemokine (C-X3-C motif) ligand 1IL10RAAdipocytokines - آدیپوسیتوکین هاS-adenosylmethionine - اس-ادنوزیل متیونینStearoyl-CoA desaturase 1 - استارویل-کاپا دساتوراز 1Fatty acids - اسیدهای چربinflammation - التهاب( توروم) interleukin - اینترلوکینinterleukin 6 - اینترلوکین 6White adipose tissue - بافت چربی سفیدGene expression - بیان ژنtransforming growth factor beta 1 - تبدیل فاکتور رشد بتا 1triglyceride - تریگلیسریدtumor necrosis factor-α - تومور نکروز عامل αtyrosine kinase 2 - تیروزین کیناز 2MIF - شهرMacrophage migration inhibitory factor - عامل مهارکننده مهاجرت ماکروفاژactivating transcription factor 2 - فعال کردن عامل رونویسی 2lipopolysaccharide - لیپوپلی ساکاریدPromoter methylation - متیلاسیون سازندهmonocyte chemoattractant protein 1 - پروتئین cheoattractant monocyte 1FAS-associated death domain protein - پروتئین دامنه مرگ مرتبط با FASGATA binding protein 3 - پروتئین گیرنده GATA 3WAT - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adipocytes are one of the primary sources of inflammatory cytokines that drive the low-grade inflammation associated with obesity and obesity-related diseases. Stearoyl-CoA desaturase, a key adipogenic enzyme in rodents and humans, plays significant role in the regulation of adipocyte inflammation via a mechanism that involves the regulation of inflammatory gene expression. In the present study, we tested the hypothesis that the stearoyl-CoA desaturase 1-related regulation of gene expression might be driven by changes in DNA methylation. We showed that stearoyl-CoA desaturase 1 overexpression causes the global hypomethylation of DNA, even as early as 12 h after the induction of differentiation, with the greatest difference seen in mature adipocytes. In contrast, both the silencing of stearoyl-CoA desaturase 1 gene expression by siRNA and inhibition of stearoyl-CoA desaturase 1 activity resulted in DNA hypermethylation in 3T3-L1 adipocytes. The analysis of the promoter methylation of 22 genes that are related to the inflammatory response showed that the level of methylation of CpG sites in interleukin-10 receptor a, interleukin-4 receptor a, interleukin-6 signal transducer, and transforming growth factor β 1 promoters was strongly related to stearoyl-CoA desaturase 1 expression or activity. The changes in methylation at CpG promoter sites correlated with differential expression of the aforementioned genes. The results show that stearoyl-CoA desaturase 1 regulates the level of DNA methylation in adipocytes and suggest that the mechanism by which stearoyl-CoA desaturase 1 affects adipocyte inflammation may involve changes in the methylation of inflammatory genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 55, October 2014, Pages 40-50
Journal: The International Journal of Biochemistry & Cell Biology - Volume 55, October 2014, Pages 40-50
نویسندگان
Malgorzata Malodobra-Mazur, Anna Dziewulska, Kamil Kozinski, Pawel Dobrzyn, Katarzyna Kolczynska, Justyna Janikiewicz, Agnieszka Dobrzyn,