کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326559 | 1539977 | 2007 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Keratinocyte serum-free medium maintains long-term liver gene expression and function in cultured rat hepatocytes by preventing the loss of liver-enriched transcription factors
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DEXcpsUGTRT-PCRKSFMPXRRetinoid X receptorRXRC/EBP - C / EBPCYPs - CYPDMSO - DMSOHnf - HNFUDP-glucuronosyltransferase - UDP-گلوکورونوسیل ترانسفرازDexamethasone - دگزامتازونDimethyl sulfoxide - دیمتیل سولفواکسیدHuman epidermal growth factor - عامل رشد اپیدرمی انسانیhepatocyte nuclear factor - عامل هسته ای هپاتوسیتHepatocyte culture - فرهنگ هپاتوسیتCAR - ماشینreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسCCAAT/enhancer-binding protein - پروتئین CCAAT / تقویت کنندهCarbamoylphosphate synthetase - کارباموییل فسفات سنتتازkeratinocyte serum-free medium - کراتینوسیت بدون سرمconnexin - کنسکسینGlutamine synthetase - گلوتامین سنتتازPregnane X receptor - گیرنده پیش گران X
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Freshly isolated hepatocytes rapidly lose their differentiated properties when placed in culture. Therefore, production of a simple culture system for maintaining the phenotype of hepatocytes in culture would greatly facilitate their study. Our aim was to identify conditions that could maintain the differentiated properties of hepatocytes for up to 28 days of culture. Adult rat hepatocytes were isolated and attached in Williams' medium E containing 10% serum. The medium was changed to either fresh Williams' medium E or keratinocyte serum-free medium supplemented with dexamethasone, epidermal growth factor and pituitary gland extract. The hepatic phenotype was then analysed using RT-PCR, immunohistochemistry, Western blotting and assays of liver function. Cells cultured in keratinocyte serum-free medium supplemented with dexamethasone, epidermal growth factor and pituitary gland extract maintained their phenotype for 3-4 weeks, based on expression of liver proteins, ureagensis and response to xenobiotics. In contrast, hepatocytes cultured in Williams' medium E rapidly lost the expression of liver proteins after 3 days. Cells cultured in keratinocyte serum-free medium supplemented with dexamethasone, epidermal growth factor and pituitary gland extract maintained their expression of liver-enriched transcription factors (C/EBPα and β, HNF4α and RXRα) while expression was either lost or reduced in cells cultured in Williams' medium E. These results suggest that keratinocyte serum-free medium supplemented with dexamethasone, epidermal growth factor and pituitary gland extract can maintain the hepatic phenotype for a prolonged period and that this is probably related to the continued expression of the liver-enriched transcription factors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 39, Issue 3, 2007, Pages 541-554
Journal: The International Journal of Biochemistry & Cell Biology - Volume 39, Issue 3, 2007, Pages 541-554
نویسندگان
Wan-Chun Li, Kate L. Ralphs, Jonathan M.W. Slack, David Tosh,