کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356282 | 1542015 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Concealed ester formation and amino acid metabolism to volatile compounds in table grape (Vitis vinifera L.) berries
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کلمات کلیدی
DEPCalcohol acetyl transferaseSPMEPAASTMMAMATAATADHDTTEtOH1,4-dithiothreitol - 1،4-dithiothreitolGC/MS - GC / MSEthanol - اتانولAlcohol acetyltransferase - استیل ترانسفراز الکلAmino acids - اسید آمینه یا آمینو اسیدAlcohol dehydrogenase - الکل دهیدروژنازFold-change - تغییر فیدPar - توسطdiethyl pyrocarbonate - دیورتیک پریکو کربناتGas Chromatography Mass Spectrometry - طیف سنجی جرم کروماتوگرافی گازSolid phase micro extraction - فاز جامد فاز استخراج میکروEnzyme activity - فعالیت آنزیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Volatile esters contribute to the aroma and flavor of many fruits but are normally absent in grape berries (Vitis vinifera L.). To examine the biosynthetic potential of grape berries to form volatile esters, berry sections were incubated with exogenous L-Phe, L-Leu or L-Met. In general, amino-acid incubation caused the accumulation of the respective aldehydes and alcohols. Moreover, L-Leu incubation resulted in the accumulation of 3-methylbutyl acetate and L-Phe incubation resulted in the accumulation 2-phenylethyl acetate in 'Muscat Hamburg' but not in the other grape accessions. Exogenous L-Met administration did not result in volatile esters accumulation but the accumulation of sulfur volatile compounds such as methional and dimethyl disulfide was prominent. Berry-derived cell-free extracts displayed differential alcohol acetyltransferase activities and supported the formation of 3-methylbutyl acetate and benzyl acetate. 2-Phenylethyl acetate was produced only in 'Muscat Hamburg' cell-free extracts. VvAAT2, a newly characterized gene, was preferentially expressed in 'Muscat Hamburg' berries and functionally expressed in E. coli. VvAAT2 possesses alcohol acetyltransferase activity utilizing benzyl alcohol, 2-phenylethanol, hexanol or 3-methylbutanol as substrates. Our study demonstrates that grape berries have a concealed potential to accumulate volatile esters and this process is limited by substrate availability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 274, September 2018, Pages 223-230
Journal: Plant Science - Volume 274, September 2018, Pages 223-230
نویسندگان
Itay Maoz, Rachel Davidovich Rikanati, Daniel Schlesinger, Einat Bar, Itay Gonda, Elena Levin, Tatiana Kaplunov, Noa Sela, Amnon Lichter, Efraim Lewinsohn,