Role of GSH homeostasis under Zn toxicity in plants with different Zn tolerance

Tripepthide glutathione (GSH) is a pivotal molecule in tolerance to heavy metals, including Zinc (Zn). The aim of our work is to examine the role of GSH metabolism in two different horticultural plants under Zn toxicity in order to select and/or generate plants tolerant to Zn toxicity. We show a comparative analysis of the toxic effect of 0.5 mM Zn between Lactuca sativa cv. Phillipus and Brassica oleracea cv. Bronco. In L. sativa the accumulation of Zn resulted in an increase in reactive oxygen species (ROS), while enzymes of GSH metabolism and the activities of the antioxidant enzymes were negatively affected. On the contrary, B. oleracea showed the existence of a detoxification mechanism of these ROS. Moreover, while in L. sativa increased the oxidized GSH (GSSG) and phytochelatins (PCs) concentration with the reduction of leaves biomass, in B. oleracea the higher concentration of reduced GSH and its use in the detoxification of ROS seems to be a major mechanism to provide tolerance to Zn toxicity without reducing leaf biomass. Our results suggested that under Zn toxicity, B. oleracea is more efficient and tolerant than L. sativa through the detoxification of lipid peroxidation products due to the reduced GSH.