کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8387742 | 1543904 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics
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کلمات کلیدی
sac.PSIIChl aExp. - ExpGC–MS - GC-MSEmiliania huxleyi - امیلیانا هاکسلیStationary - ثابتmaximum fluorescence - حداکثر فلورسنسDiploid - دیپلیدRetention time - زمان بازداریSaccharide - ساخاریدgas chromatography–mass spectrometry - طیف سنجی جرم کروماتوگرافی گازNumber - عددPhotosystem II - فتوسیستم 2initial fluorescence - فلورسانس اولیهPhytoplankton - فیتوپلانکتون هاMetabolite - متابولیتMetabolites - متابولیت هاMethyl ester - متیل استرDerivative - مشتقExponential - نمایشیNo. - نهHaploid - هپلوئیدCaP - کلاه لبه دارChlorophyll a - کلروفیل aComplex - کمپلکسCoccolithophore - کوکولیتوفور
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics](/preview/png/8387742.png)
چکیده انگلیسی
In phytoplankton a high species diversity of microalgae co-exists at a given time. But diversity is not only reflected by the species composition. Within these species different life phases as well as different metabolic states can cause additional diversity. One important example is the coccolithophore Emiliania huxleyi. Diploid cells play an important role in marine ecosystems since they can form massively abundant algal blooms but in addition the less abundant haploid life phase of E. huxleyi occurs in lower quantities. Both life phases may fulfill different functions in the plankton. We hypothesize that in addition to the functional diversity caused by this life phase transition the growth stage of cells can also influence the metabolic composition and thus the ecological impact of E. huxleyi. Here we introduce a metabolomic survey in dependence of life phases as well as different growth phases to reveal such changes. The comparative metabolomic approach is based on the extraction of intracellular metabolites from intact microalgae, derivatization and analysis by gas chromatography coupled to mass spectrometry (GC-MS). Automated data processing and statistical analysis using canonical analysis of principal coordinates (CAP) revealed unique metabolic profiles for each life phase. Concerning the correlations of metabolites to growth phases, complex patterns were observed. As for example the saccharide mannitol showed its highest concentration in the exponential phase, whereas fatty acids were correlated to stationary and sterols to declining phase. These results are indicative for specific ecological roles of these stages of E. huxleyi and are discussed in the context of previous physiological and ecological studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 172, 1 January 2015, Pages 137-148
Journal: Journal of Plant Physiology - Volume 172, 1 January 2015, Pages 137-148
نویسندگان
Michaela A. Mausz, Georg Pohnert,