کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8451045 | 1547692 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Common traffic routes for imported spermine and endosomal glypican-1-derived heparan sulfate in fibroblasts
ترجمه فارسی عنوان
مسیرهای ترافیکی مشترک برای هپارین سولفات مشتق شده اسپرمین و گلیپیکن اندوزومی در فیبروبلاست ها
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کلمات کلیدی
anhydromannoseGlypican-1DFMOα-DifluoromethylornithineAutophagosomesGPC-1Tg2576S-nitrosothiolSNONCLAPPDAPISPMAβMEFDMEMAscorbate - آسکوربات یا ویتامین ثSpermine - اسپرمminimal essential medium - حداقل وسایل ضروریMEM - مامانmouse embryonic fibroblasts - موش فیبروبلاست جنینیwild-type - نوع وحشیNucleolin - نولولینNitric oxide - نیتریک اکسیدHeparan sulfate - هپاران سولفاتamyloid precursor protein - پروتئین پیش ماده آمیلوئیAmyloid beta peptides - پپتیدهای بتا آمیلئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Import of the polyamine spermine from the extracellular environment depends on the presence of cell surface heparan sulfate proteoglycans, such as glypican-1. This proteoglycan is internalized by endocytosis, releases its heparan sulfate chains in endosomes by a nitric oxide-, copper- and amyloid precursor protein-dependent mechanism, then penetrates the membrane and is transported to the nucleus and then to autophagosomes. This process is spontaneous or induced by ascorbate depending on the growth-state of the cell. Here, we have explored possible connections between the heparan sulfate traffic route and spermine uptake and delivery in wild-type and Tg2576 mouse fibroblasts. Cells were examined by deconvolution immunofluorescence microscopy. The antibodies used were specific for spermine, glypican-1-derived heparan sulfate, Rab7, nucleolin and a marker for autophagosomes. Endogenous immunostainable spermine was primarily associated with autophagosomes. When spermine synthesis was inhibited, imported spermine appeared in Rab7-positive endosomes. When ascorbate was added, heparan sulfate and spermine were transported to the nucleus where they colocalized with nucleolin. Spermine also appeared in autophagosomes. In a pulse-chase experiment, heparan sulfate and spermine were first arrested in late endosomes by actinomycin D treatment. During the chase, when arrest was abolished, heparan sulfate and spermine were both transported to the nucleus and targeted nucleolin. In amyloid precursor protein-/--fibroblasts, ascorbate failed to induce release of heparan sulfate and spermine remained in the endosomes. We propose that cell surface glypican-1 carries spermine to the endosomes and that the released heparan sulfate carries spermine across the membrane into the cytosol and then to the nucleus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 364, Issue 2, 15 March 2018, Pages 133-142
Journal: Experimental Cell Research - Volume 364, Issue 2, 15 March 2018, Pages 133-142
نویسندگان
Fang Cheng, Lars-Ã
ke Fransson, Katrin Mani,