کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8525451 | 1557940 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Astragalus polysaccharide protects hypoxia-induced injury by up-regulation of miR-138 in rat neural stem cells
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کلمات کلیدی
PVDFHRPqRT-PCRECLbFGFNSCsHIEmRNADMEMPBSmiRNA/miRGSCsEGFNeural stem cells (NSCs)CCK-8PASMCsAPs - AP هاBSA - BSAmessenger RNA - RNA messengerbovine serum albumin - آلبومین سرم گاوHypoxic-ischemic encephalopathy - آنسفالوپاتی ناشی از هیپوکسی-ایسکمیکCell apoptosis - آپوپتوز سلولیSprague-Dawley - اسپراگ داولیSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدmiR-138 - به miR-138enhanced chemiluminescence - بهبود شیمیایی لومنANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههCNS - دستگاه عصبی مرکزیGlioma stem cells - سلول های بنیادی گلیوماpulmonary artery smooth muscle cells - سلول های عضلانی عروق قلب ریویNeural stem cells - سلولهای بنیادی عصبیcentral nervous system - سیستم عصبی مرکزیcell counting kit-8 - شمارش سلول کیت 8epidermal growth factor - عامل رشد اپیدرمیPolyvinylidene fluoride membrane - غشای فلوراید پلی وینیلیدینbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoMicroRNA - میکرو RNA Hypoxia - هیپوکسیQuantitative reverse transcriptase-polymerase chain reaction - واکنش زنجیره ای ترانس کریتاز - پلیمریزا معکوسHorseradish peroxidase - پراکسیداز هوررادیشPropidium iodide - پروتئین یدیدAstragalus polysaccharide - پلی ساکارید Astragalus
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Astragalus polysaccharide protects hypoxia-induced injury by up-regulation of miR-138 in rat neural stem cells Astragalus polysaccharide protects hypoxia-induced injury by up-regulation of miR-138 in rat neural stem cells](/preview/png/8525451.png)
چکیده انگلیسی
Astragalus polysaccharide (APS) is the main active ingredient of astragalus and exhibits various pharmacological effects. This study aimed to investigate the effect of APS on hypoxia-induced injury in neural stem cells (NSCs). The NSCs derived from the hippocampus of rat were subjected to hypoxia incubator to establish a hypoxia model. NSCs were pretreated with APS before hypoxia injury to investigate the effect of APS. The expression of miR-138 was inhibited by transfection with miR-138 inhibitor and the miR-138 level was measured by qRT-PCR. Cell viability and apoptotic cell rates were respectively assessed by CCK-8 assay and flow cytometry assay. Western blot was performed to determine the expression of apoptosis-, JNK pathway- and p38MAPK pathway-related factors. Hypoxia exposure caused the reduction of cell viability and induction of cell apoptosis of NSCs. However, APS pretreatment attenuated the cell injury induced by hypoxia, as evidenced by increased cell viability, reduced apoptotic cells, inhibited expression of pro-apoptotic factors and enhanced expression of anti-apoptotic factor. Interestingly, higher miR-138 expression was observed in the hypoxia-injured NSCs compared with normoxia, and miR-138 expression was further up-regulated by APS pretreatment. Furthermore, miR-138 inhibitor blocked the protective effect of APS on hypoxia-injured NSCs. In addition, we found that APS inhibited the JNK and p38MAPK pathways through miR-138. In conclusion, this study demonstrated a protective effect of APS on hypoxia-induced NSC injury. The regulatory mechanism might be mediated by up-regulation of miR-138 and inhibition of the JNK and p38MAPK pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 102, June 2018, Pages 295-301
Journal: Biomedicine & Pharmacotherapy - Volume 102, June 2018, Pages 295-301
نویسندگان
Zebao Zheng, Bing Zhao,