کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553415 | 1562586 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Brain damage and neurological symptoms induced by T-2 toxin in rat brain
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
5-HTBPbTDI5-hydroxytryptamineIFN-γRIPAMMP-9RT-qPCRMAOi.g.ATAmTORLC3BATG5GAPDHESIPBSAtg14DABLD50LSDKBDmRNAMatrix metalloproteinases 9BSA - BSADMSO - DMSOHT-2 toxin - HT-2 توکسینLC-MS/MS - LC-MS / MSmessenger RNA - RNA messengerRadio immunoprecipitation assay - آزمایش ایمنی ادرار رادیوییbovine serum albumin - آلبومین سرم گاوSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدTem - این استinterferon-gamma - اینترفرون گاماinterleukin - اینترلوکینBax - باکسANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههintragastric administration - تزریق داخل عضلانیLeast Significance Difference - تفاوت معنایی کمترینT-2 toxin - توکسین T-2Domain antibody - دامنه آنتی بادیCNS - دستگاه عصبی مرکزیlethal dose 50 - دوز مرگبار 50blood brain barrier - سد خونی مغزیBBB - سد خونی مغزیHuman health - سلامتی انسانNeurotoxicity - سمیت عصبیcentral nervous system - سیستم عصبی مرکزیPhosphate buffered saline - فسفات بافر شورGray matter - ماده خاکستریwhite matter - ماده سفیدDaily intake - مصرف روزانهBrain - مغزmonoamine oxidase - مونوآمین اکسیدازها Transmission electron microscope - میکروسکوپ الکترونی انتقالMechanistic target of rapamycin - هدف مکانیکی رپامایسینhematoxylin-eosin - هماتوکسیلین ائوزینHypophysis - هیپوفیزquantitative real time-polymerase chain reaction - واکنش زنجیره ای زمان واقعی-پلیمراز کمیmicrotubule-associated protein 1 light chain 3 beta - پروتئین مرتبط با میکروتوبول 1 زنجیره سبک 3 بتاCaspase-3 - کاسپاز ۳ Caspase-9 - کاسپاز-9glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
T-2 toxin, a trichothecene mycotoxin, is a common contaminant in food and animal feed, and is also present in processed cereal products. The most common route of T-2 toxin exposure in humans is through dietary ingestion. The cytotoxic effects of T-2 toxin include modifications to feeding behavior, nervous disorders, cardiovascular alterations, immunosuppression, and hemostatic derangements. However, to date, effects on the central nervous system (CNS) have rarely been reported. In the present study, female Wistar rat were given a single dose of T-2 toxin at 2â¯mg/kg b.w. and were sacrificed at one, three, and seven days post-exposure. Histopathological analysis and transmission electron microscope (TEM) observations were used to investigate injury to the brain and pituitary gland. Damage to the brain and pituitary at the molecular level was detected by real time-polymerase chain reaction (RT-PCR), western blot, and immunohistochemical assays. Liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS) was used to investigate T-2 concentration in the brain. The results showed that pathological lesions were obvious in the brain at three days post-exposure; lesions in the pituitary were not observed until seven days post-exposure. Autophagy in the brain and apoptosis in the pituitary suggest that T-2 toxin may induce different acute reactions in different tissues. Importantly, low concentrations of T-2 toxin in the brain were observed in only one rat. Responsible for the above mentioned, we hypothesize that brain damage caused by this toxin may be due to the ability of the toxin to directly cross the blood-brain barrier (BBB). Therefore, given its widespread pollution in food, we should pay more attention to the neurotoxic effects of the T-2 toxin, which may have widespread implications for human health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 286, April 2018, Pages 96-107
Journal: Toxicology Letters - Volume 286, April 2018, Pages 96-107
نویسندگان
Pu Guo, Aimei Liu, Deyu Huang, Qinghua Wu, Zainab Fatima, Yanfei Tao, Guyue Cheng, Xu Wang, Zonghui Yuan,