کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8715892 | 1587874 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Propionibacterium acnes-Derived Extracellular Vesicles Promote Acne-Like Phenotypes in Human Epidermis
ترجمه فارسی عنوان
فسیل های پروپیونی باکتریوم آکنه در خارج از سلول ها به عنوان فنوتیپ های آکنه در اپیدرم انسان موثرند
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کلمات کلیدی
PBMCTLRPEVFLGNHEKDSC1MAPK - MAPKSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAToll-like receptor - تیالآرPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیfilaggrin - فیلهگرینextracellular vesicle - ویسکوز خارج سلولیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenkeratin 10 - کراتین 10Normal human epidermal keratinocyte - کراتینوسیت اپیدرمی طبیعی انسان
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
چکیده انگلیسی
Acne vulgaris is an inflammatory disease occurring in the pilosebaceous unit and is the most common skin condition in young people. A gram-positive bacterium, Propionibacterium acnes, has been suspected to contribute to the development of acne. Here, we report that P. acnes constitutively releases extracellular vesicles (EVs) exhibiting typical EV morphology and size. Moreover, the P. acnes-derived EVs (PEVs) can induce acne-like phenotypes in human epidermal keratinocytes and a reconstituted human skin model. PEVs significantly induced inflammatory cytokines IL-8 and GM-CSF and dysregulated epidermal differentiation by increasing proliferating keratinocytes and decreasing epidermal keratin 10 and desmocollin 1 levels. PEVs showed strong effects, evoking these responses at earlier time points compared with P. acnes extract at the same protein concentration. We verified that PEVs were internalized via clathrin-dependent endocytosis into keratinocytes and that PEV-induced cellular responses occurred via Toll-like receptor 2-dependent signal cascades. Furthermore, PEVs showed a stronger effect than keratinocytes in inducing inflammatory cytokines in myeloid cells. Collectively, our study suggests that PEVs induce acne-like phenotypes in a unique way; therefore, inhibiting the release of EVs from P. acnes or targeting PEV-mediated signaling pathways could represent an alternative method for alleviating acne occurrence and phenotypes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 138, Issue 6, June 2018, Pages 1371-1379
Journal: Journal of Investigative Dermatology - Volume 138, Issue 6, June 2018, Pages 1371-1379
نویسندگان
Eun-Jeong Choi, Hyun Gee Lee, Il-Hong Bae, Wanil Kim, Jungwon Park, Tae Ryong Lee, Eun-Gyung Cho,