کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8715940 | 1587875 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced Repair of UV-Induced DNA Damage by 1,25-Dihydroxyvitamin D3 in Skin Is Linked to Pathways that Control Cellular Energy
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کلمات کلیدی
ECARUDSmTORC1PARP8-oxo-dGOCRGSK3ΔΨm2-deoxy-d-glucoseacutely transforming retrovirus AKT8 in rodent T-cell lymphomaMDVUVRAG1α,25-dihydroxyvitamin D3 - 1α، 25-دی هیدروکسوییتامین D31,25(OH)2D3 - 1،25 (OH) 2D32-DG - 2 DG8-oxo-2′-deoxyguanosine - 8-اکسو-2'-دگزسی گوانوزینcpd - CPDERK1/2 - ERK1 / 2ROS - ROSAkt - آکتcyclobutane pyrimidine dimer - دییریر پیریمیدین cyclobutaneUnscheduled DNA synthesis - سنتز DNA بدون برنامه ریزی شدهOxidative phosphorylation - فسفوریلاسیون اکسیداتیوOxygen consumption rate - میزان مصرف اکسیژنextracellular acidification rate - نرخ اسیدی شدن خارج سلولیMammalian target of rapamycin complex 1 - هدف پستانداران مجتمع رپامایسین 1Mitochondrial membrane potential - پتانسیل غشای میتوکندریPoly(ADP-ribose) polymerase-1 - پلی (ADP-ribose) پلیمراز-1glycogen synthase kinase-3 - گلیکوزین سنتاز کیناز 3Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
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چکیده انگلیسی
Inadequately repaired post-UV DNA damage results in skin cancers. DNA repair requires energy but skin cells have limited capacity to produce energy after UV insult. We examined whether energy supply is important for DNA repair after UV exposure, in the presence of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), which reduces UV-induced DNA damage and photocarcinogenesis in a variety of models. After UV exposure of primary human keratinocytes, the addition of 1,25(OH)2D3 increased unscheduled DNA synthesis, a measure of DNA repair. Oxidative phosphorylation was depleted in UV-irradiated keratinocytes to undetectable levels within an hour of UV irradiation. Treatment with 1,25(OH)2D3 but not vehicle increased glycolysis after UV. 2-Deoxyglucose-dependent inhibition of glycolysis abolished the reduction in cyclobutane pyrimidine dimers by 1,25(OH)2D3, whereas inhibition of oxidative phosphorylation had no effect. 1,25(OH)2D3 increased autophagy and modulated PINK1/Parkin consistent with enhanced mitophagy. These data confirm that energy availability is limited in keratinocytes after exposure to UV. In the presence of 1,25(OH)2D3, glycolysis is enhanced along with energy-conserving processes such as autophagy and mitophagy, resulting in increased repair of cyclobutane pyrimidine dimers and decreased oxidative DNA damage. Increased energy availability in the presence of 1,25(OH)2D3 is an important contributor to DNA repair in skin after UV exposure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 138, Issue 5, May 2018, Pages 1146-1156
Journal: Journal of Investigative Dermatology - Volume 138, Issue 5, May 2018, Pages 1146-1156
نویسندگان
Mark Stephen Rybchyn, Warusavithana Gunawardena Manori De Silva, Vanessa Bernadette Sequeira, Bianca Yuko McCarthy, Anthony Vincent Dilley, Katie Marie Dixon, Gary Mark Halliday, Rebecca Sara Mason,