کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002074 | 1118571 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Morin inhibits 12-O-tetradecanoylphorbol-13-acetate-induced hepatocellular transformation via activator protein 1 signaling pathway and cell cycle progression
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کلمات کلیدی
ATCCEMSAp-iodonitrotetrazolium violetrelative luciferase unitJNKsDulbecco Modified Eagle MediumRLUAP-1int12-O-tetradecanoylphorbol-13-acetatetPADMEMFBS3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromidec-Jun N-terminal kinases - C-Jun N-terminal kinasesERKs - ERK هاMTT - MTTElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزTransformation - دگرگونیfetal bovine serum - سرم جنین گاوAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییMorin - مورینmap - نقشهHepatocyte - هپاتوسیتmitogen-activated protein - پروتئین فعال mitogenactivator protein 1 - پروتئین فعال کننده 1mitogen-activated protein kinase - پروتئین کیناز فعال با mitogenCell cycle - چرخه سلولیextracellular signal-regulated kinases - کیناز های تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Flavonoids are constituents of fruits, vegetables, and plant-derived beverages, as well as components in herbal containing dietary supplements. They exhibit a remarkable spectrum of biochemical and pharmacological activities. In this study, we examined morin (3,5,7,2â²,4â²-pentahydroxyflavone) for its effect on 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated human hepatocytes. Morin inhibited TPA-induced cellular transformation in Chang liver cells in a dose-dependent manner. Luciferase assay and electrophoretic mobility shift assay revealed that morin suppressed TPA-induced AP-1 activity, and the inhibition of AP-1 activity by morin was mediated through the inhibition of p38 kinase. Moreover, morin induced the S-phase arrest and inhibited the DNA synthesis in TPA-treated hepatocytes, suggesting that a cell cycle checkpoint was activated by morin to block DNA synthesis in S phase. In conclusion, our results suggested that morin was a potent anti-hepatocellular transformation agent that inhibited cellular transformation by suppressing the AP-1 activity and inducing the S-phase arrest in human hepatocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 11, 1 June 2005, Pages 1603-1611
Journal: Biochemical Pharmacology - Volume 69, Issue 11, 1 June 2005, Pages 1603-1611
نویسندگان
Chien-Yun Hsiang, Shih-Lu Wu, Tin-Yun Ho,