کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9002235 | 1118578 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spin adduct formation from lipophilic EMPO-derived spin traps with various oxygen- and carbon-centered radicals
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کلمات کلیدی
DMPOEMPOPBN5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxideDEPMPOHFS5,5-dimethylpyrroline-N-oxideDTPAnuclear magnetic resonance - رزونانس مغناطیسی هستهایN-tert-butyl-α-phenylnitrone - N-tert-butyl-a-phenylnitroneO2− - O2-NMR - تشدید مغناطیسی هستهای Electron paramagnetic resonance - تشدید پارامغناطیس الکترونEPR - تشدید پارامغناطیس الکترونHyperfine splitting - تقسیم HyperfineSpin traps - تله چرخشDiethylenetriaminepentaacetic acid - دی اتیلنتریمین پنتا اسیدهای اسیدFree radicals - رادیکال آزادSuperoxide anion radical - رادیکال آنیون سوپراکسیدSOD - سدSuperoxide - سوپر اکسیدSuperoxide dismutase - سوکسوکس دیسموتازLinoleic acid hydroperoxide - هیدروپراکسید اسید لینولئیک
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Free radicals are involved in the onset of many diseases, therefore the availability of adequate spin traps is crucial to the identification and localization of free radical formation in biological systems. In recent studies several hydrophilic compounds of 2-ethoxycarbonyl-2-methyl-pyrroline-N-oxide (EMPO) have been found to form rather stable superoxide spin adducts with half-lives up to twenty minutes at physiological pH. This is a major improvement over DMPO (t1/2Â =Â ca. 45Â s), and even over DEPMPO (t1/2Â =Â ca. 14Â min), the best commercially available spin trap for the unambiguous detection of superoxide radicals. In order to allow the detection of superoxide and also other radicals in lipid environment a series of more lipophilic derivatives of EMPO was synthesized and their structure unambiguously characterized by 1H and 13C NMR spectroscopy. In this way, six different compounds with a n-butyl group in position 5 and either an ethoxy- (EBPO), propoxy- (PBPO), iso-propoxy- (iPBPO), butoxy- (BBPO), sec-butoxy- (sBBPO) or tert-butoxycarbonyl group (tBBPO) in position 5 of the pyrroline ring were obtained and fully analytically characterized (NMR, IR). The stability of the superoxide adducts of all investigated spin traps were comparable with EMPO (t1/2Â =Â ca. 8Â min), except for the two compounds bearing an additional methyl group in position 3 or 4 of the pyrroline ring, 5-butyl-5-ethoxycarbonyl-3-methyl-pyrroline-N-oxide (BEMPO-3) and 5-butyl-5-ethoxycarbonyl-4-methyl-pyrroline-N-oxide (BEMPO-4), of which the superoxide adducts were stable for more than 30Â min. Spin adducts of other carbon- and oxygen-centered radicals were also investigated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 69, Issue 2, 15 January 2005, Pages 297-305
Journal: Biochemical Pharmacology - Volume 69, Issue 2, 15 January 2005, Pages 297-305
نویسندگان
Klaus Stolze, Natascha Udilova, Thomas Rosenau, Andreas Hofinger, Hans Nohl,