Cystatin A inhibits IL-8 production by keratinocytes stimulated with Der p 1 and Der f 1: Biochemical skin barrier against mite cysteine proteases

The proteolytic activity of Der p 1 and Der f 1 stimulates human keratinocytes in vitro. Cystatin A produced by keratinocytes is the dominant biochemical skin barrier that eliminates the enzymatic activity of these mite cysteine proteases and prevents them from stimulating keratinocytes.