کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9282892 | 1593662 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antibody responses to individual proteins of SARS coronavirus and their neutralization activities
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کلمات کلیدی
SARS-CoVTCID50SARS coronavirusS proteinantibodies - آنتی بادی هاNeutralizing antibodies - آنتی بادیهای ناتریالProtein microarray - ریزپردازنده پروتئینFluorescence intensity - شدت فلورسانسE protein - پروتئین EM protein - پروتئین MN protein - پروتئین NNucleocapsid protein - پروتئین NucleocapsidMembrane protein - پروتئین غشائیRecombinant protein - پروتئین های نوترکیبSpike glycoprotein - گلیکوپروتئین اسپایکEnvelope glycoprotein - گلیکوپروتئین پاکت
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Antibody responses to individual proteins of SARS coronavirus and their neutralization activities Antibody responses to individual proteins of SARS coronavirus and their neutralization activities](/preview/png/9282892.png)
چکیده انگلیسی
A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 7, Issues 5â6, May 2005, Pages 882-889
Journal: Microbes and Infection - Volume 7, Issues 5â6, May 2005, Pages 882-889
نویسندگان
Maofeng Qiu, Yuling Shi, Zhaobiao Guo, Zeliang Chen, Rongqiao He, Runsheng Chen, Dongsheng Zhou, Erhei Dai, Xiaoyi Wang, Bingyin Si, Yajun Song, Jingxiang Li, Ling Yang, Jin Wang, Hongxia Wang, Xin Pang, Junhui Zhai, Zongmin Du, Ruifu Yang,