کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9774631 | 1509090 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Induction of apoptosis in macrophages by air oxidation of dioleoylphosphatidylglycerol
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کلمات کلیدی
zVAD-fmkDOPGMALDI-TOFPKCDHB2,5-dihydroxybenzoic acid - 2،5-دی هیدروکسی بنزوئیک اسیدdioleoylphosphatidylglycerol - dioleoyl فسفاتیدیل گلیسرولMAPKs - MAPK هاOxidation - اکسیداسیونbenzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone - بنزیلوکسیکربنیل-وال-آلا-آسپ-فلورومتیلیکونApoptosis - خزان یاختهایCytotoxicity - سمیت سلولیmononuclear phagocyte system - سیستم فاگوسیتی تک هسته ایLOOH - لئوlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Macrophages - ماکروفاژها،درشت خوارهاMPs - نمایندگان مجلسLipid hydroperoxide - هیدروپراکسید لیپیدProtein kinase C - پروتئین کیناز سیPropidium iodide - پروتئین یدیدmitogen-activated protein kinases - کیناز پروتئین فعال Mitogen
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dioleoylphosphatidylglycerol (DOPG) containing unsaturated sites is the target of oxidation during preparation, storage, or in vivo use of anionic liposomes. We investigated the biological effect of air oxidation of DOPG on RAW 264.7 murine macrophage-like cells. Oxidation was induced by exposing DOPG to air for 24-72 h. The extent of air oxidation was confirmed using Matrix-Assisted Laser Desorption and Ionization with Time-of-Flight (MALDI-TOF) mass spectrometry. The product of the air oxidation of DOPG was identified as the addition of one oxygen atom to one of the symmetrical fatty moieties of DOPG at m/z 814.77. The treatment of DOPG with air oxidation produced dose-dependent cytotoxicity in macrophages. RAW 264.7 cells exposed to oxidized DOPG exhibited morphological features of apoptosis, such as chromatin condensation and cell shrinkage. Typical apoptotic ladders were observed in DNA extracted from RAW 264.7 cells treated with oxidized DOPG. Flow cytometric analysis demonstrated an increase in the hypodiploid DNA population (sub-G1), indicating that DNA cleavage occurred after treatment with oxidized DOPG. In addition, we showed that pretreating RAW 264.7 cells with zVAD-fmk, a general caspase inhibitor, did not prevent apoptosis induced by oxidized DOPG, suggesting that apoptosis in macrophage cells follows a caspase-independent pathway. These results point to a need for precaution in formulating DOPG liposomes for drug delivery and therapeutic purposes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 108, Issues 2â3, 28 November 2005, Pages 442-452
Journal: Journal of Controlled Release - Volume 108, Issues 2â3, 28 November 2005, Pages 442-452
نویسندگان
Jung-hua Steven Kuo, Ming-shiou Jan, Jingyueh Jeng, Hsuan Wen Chiu,