کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9887797 | 1538439 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aptamers against extracellular targets for in vivo applications
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کلمات کلیدی
ECM18F99mTcPrPcssDNAPDGFPrPPrPscAChRLNAAMDSELEXFDASingle strand DNA - DNA تک رشته ایAptamers - آپتامرزLocked Nucleic Acid - اسید نوکلئیک قفل شدهHER - اوDiagnostic - تشخیصPositron emission tomography - توموگرافی گسیل پوزیترونsystematic evolution of ligands by exponential enrichment - تکامل سیستماتیک لیگاند ها با غنی سازی نمایشیRET - حقRearranged during transfection - در ترانسفکشن دوباره تنظیم شده استTherapeutic - درمانیIn vivo - درون تنی یا درونجانداریdiacylglycerol - دیسیل گلیسیرینDAG - روزFood and Drug Administration - سازمان غذا و داروage-related macular degeneration - سن تخریب ماکولا مربوط به سن استVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)platelet-derived growth factor - فاکتور رشد حاصل از پلاکتFluorine 18 - فلور 18Extracellular matrix - ماتریکس خارج سلولیMyasthenia gravis - میاستنی گراویسhemagglutinin - هماگلوتینینPET - پتPrion protein - پروتئین پریونpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول Purine - پورینPyrimidine - پیریمیدینacetylcholine receptor - گیرنده استیل کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Oligonucleotides are multifunctional molecules which can interfere with gene expression by different mechanism such as antisense, RNA interference, ribozymes, etc. For most in vivo diagnostic and therapeutic applications, oligonucleotides need to be delivered to the intracellular compartment of a specific organ, a difficult task which limits considerably their use. However, aptamer oligonucleotides which target extracellular markers obviate this problem. Aptamers are short oligonucleotides (< 100 bases) selected from large combinatorial pools of sequences for their capacity to bind to many types of different targets, ranging from small molecules (amino acids, antibioticsâ¦) to proteins or nucleic acid structures. Aptamers present the same high specificity and affinity for their targets as antibodies. In addition to efficient binding, aptamers have been shown in many cases to display an inhibitory activity on their targets. Moreover, they seem to lack immunogenicity and can be chemically modified in order to improve their stability against nucleases or extend their blood circulation time, two properties which are particularly useful for in vivo applications. Recently, aptamers have been selected against whole living cells, opening a new avenue which presents three major advantages 1) direct selection without prior purification of the targets; 2) conservation of membrane proteins in their native conformation similar to the in vivo conditions and 3) identification of (new) targets for a specific phenotype. Many aptamers are now being developed against biomedical relevant extracellular targets: membrane receptor proteins, hormones, neuropeptides, coagulation factors⦠Among them, one aptamer that inhibits the human VEGF165 has recently been approved by FDA for the treatment of age-related macular degeneration. Here we discuss the recent developments of aptamers against extracellular targets for in vivo therapy and as tools for diagnosis using molecular imaging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 87, Issues 9â10, SeptemberâOctober 2005, Pages 921-930
Journal: Biochimie - Volume 87, Issues 9â10, SeptemberâOctober 2005, Pages 921-930
نویسندگان
C. Pestourie, B. Tavitian, F. Duconge,