کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9989762 | 1580765 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Triptolide protects dopaminergic neurons from inflammation-mediated damage induced by lipopolysaccharide intranigral injection
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کلمات کلیدی
PPARγNonidet P-401-methyl-4-phenylpyridinium ion1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridineNP40CR3phorbol 12-myristate 13-acetateDOPACMPTPPMSF6-OHDAiNOSCOX-2PBSLPS6-HydroxydopaminePMA - LDC هاMPP+ - MPP +interferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینParkinson's disease - بیماری پارکینسونELISA - تست الیزاCNS - دستگاه عصبی مرکزیDopamine - دوپامینdihydroxyphenylacetic acid - دی هیدروکسی فنیل اسیدهای اسیدinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییcentral nervous system - سیستم عصبی مرکزیcyclooxygenase 2 - سیکلوکوکسیژناز 2phenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدlipopolysaccharide - لیپوپلی ساکاریدMHC - مجموعه سازگاری بافتی اصلیmajor histocompatibility complex - مجموعه سازگاری بافتی اصلیNitric oxide - نیتریک اکسیدoptical density - چگالی نوریHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراComplement receptor 3 - گیرنده تکمیلی 3
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Triptolide protects dopaminergic neurons from inflammation-mediated damage induced by lipopolysaccharide intranigral injection Triptolide protects dopaminergic neurons from inflammation-mediated damage induced by lipopolysaccharide intranigral injection](/preview/png/9989762.png)
چکیده انگلیسی
Converging lines of evidence suggest that neuroinflammatory processes may account for the progressive death of dopaminergic neurons in Parkinson's disease (PD). Therefore, anti-inflammatory strategies have attracted much interest for their potential to prevent further deterioration of PD. Our previous study showed that triptolide, a traditional Chinese herbal compound with anti-inflammatory and immunosuppressive properties, protected dopaminergic neurons from lipopolysaccharide (LPS)-induced damage in primary embryonic midbrain cell cultures. To examine further if triptolide can protect dopaminergic neurons from inflammation-mediated damage in vivo, microglial activation and injury of dopaminergic neurons were induced by LPS intranigral injection, and the effects of triptolide treatment on microglial activation and survival ratio and function of dopaminergic neurons were investigated. Our results demonstrated that microglial activation induced by a single intranigral dose of 10 μg of LPS reduced the survival ratio of tyrosine hydroxylase-immunoreactive (TH-ir) neurons in the substantia nigra pars compacta (SNpc) to 29% and the content of dopamine (DA) in striatum to 37% of the non-injected side. Intriguingly, treatment with triptolide of 5 μg/kg for 24 days once per day dramatically improved the survival rate of TH-ir neurons in the SNpc to 79% of the non-injected side. Meanwhile, treatment with triptolide of 1 or 5 μg/kg for 24 days once per day significantly improved DA level in striatum to 70% and 68% of the non-injected side, respectively. Complement receptor 3 (CR3) immunohistochemical staining revealed that triptolide treatment potently inhibited LPS-elicited deleterious activation of microglia in SNpc. The excessive production of cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-1β, was significantly abolished by triptolide administration. These results, together with our previous data in vitro, highly suggest the effectiveness of triptolide in protecting dopaminergic neurons against inflammatory challenge.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 18, Issue 3, April 2005, Pages 441-449
Journal: Neurobiology of Disease - Volume 18, Issue 3, April 2005, Pages 441-449
نویسندگان
Hui-Fang Zhou, Xian-Yu Liu, Dong-Bin Niu, Feng-Qiao Li, Qi-Hua He, Xiao-Min Wang,