
Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E
Keywords: مس; CD; circular dichroism; CSM; center of fluorescence spectral mass; DHF; dihydrofolate; DHFR; dihydrofolate reductase; HSQC; heteronuclear single quantum correlation; MES; 2-(N-morpholino)ethanesulfonic acid; MTE; 50Â mM MES, 25Â mM Tris 25Â mM ethanolamin