کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559763 | 969670 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of monoclonal antibodies against hop-derived (Humulus lupulus L.) prenylflavonoids and the development of immunoassays
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کلمات کلیدی
EquolFPLCIC50TLCMWCOMSDENLNHSDCCTMBHmpATEMEDDMFo-phenylenediamine dihydrochloridepNPPpara-nitrophenylphosphateXanthohumolN-hydroxysuccinimidedicyclohexylcarbodiimide8-prenylnaringenin6-prenylnaringenin8-PNDMXFBSSDSDMEMABTSPBS17β-estradiol - 17β استرادیول1H and 13C NMR - 1H و 13C NMR3,3′,5,5′-tetramethylbenzidine - 3،3 '، 5،5'-تترامیلیل بنزیدینBSA - BSADMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده Dulbeccoenterodiol - EnterodiolN,N,N′,N′-tetramethylethylenediamine - N، N، N '، N'-tetramethylethylenediamineopd - UPDbovine serum albumin - آلبومین سرم گاوUrine - ادرار enterolactone - انتولوکتونisoxanthohumol - ایزوکسون هومولELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاDaidzein - داایدزینdimethylformamide - دی متیل فرمالیدDimethylsulfoxide - دیمتیل سولفواکسیدSerum - سرمfetal bovine serum - سرم جنین گاوfast protein liquid chromatography - سریع کروماتوگرافی مایع پروتئینCoefficient of Variation - ضریب تغییرPearson's correlation coefficient - ضریب همبستگی پیرسونMass spectrometry - طیف سنجی جرمیGenistein - ماده جنستئینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMHz - مگا هرتزmegahertz - مگا هرتزMolecular Weight Cut Off - وزن مولکولی کاهش یافته استMonoclonal antibodies - پادتنهای تَکتیرهend - پایانpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول GEN - ژنرالthin-layer chromatography - کروماتوگرافی نازک لایهGas chromatography - کروماتوگرافی گازیHAT - کلاهnegative control - کنترل منفیquality control - کنترل کیفیتCoumestrol - کومسترول
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Monoclonal antibodies against the hop-derived prenylated chalcone xanthohumol (X) and the prenylated flavonoids isoxanthohumol (IX) and 8-prenylnaringenin (8-PN) were developed. Carboxylic acid haptens of X, IX and 8-PN were synthesized by linking a spacer to their C4â²-OH group followed by subsequent coupling to bovine serum albumin (BSA) to form conjugates that were employed as immunogens in BALB/c mice to raise antibodies. The monoclonal antibodies that were secreted from the established hybridoma cell lines proved, in cross-reactivity studies, to possess highly specific binding capacities in an optimized competitive indirect ELISA. The immunoassays make use of immunogen-coated microtiterplates and a peroxidase-labeled anti-mouse IgG1 secondary antibody with ABTS as a chromogenic substrate. For X the IC50 value derived from the standard curve was 62.91 ng mLâ1, and for both IX and 8-PN 37.15 ng mLâ1. The assay was validated for the quantitative analysis of X, IX and 8-PN in urine and serum. A simple sample pretreatment procedure using a diethyl ether extraction was optimized and the recoveries and matrix effects were assessed. The validity of the established assay was tested and mean inter- and intra-assay variations in urine were 2.32% and 1.91%, respectively for X, 6.24% and 2.39%, respectively for IX and 7.18% and 0.74%, respectively for 8-PN. In serum, the mean inter- and intra-assay variations were 8.90% and 1.37%, respectively for X, 6.13% and 1.57%, respectively for IX and 6.13% and 2.43%, respectively for 8-PN. Furthermore, the method demonstrated excellent accuracy and significant correlation with measurements by an established and validated HPLC-MS method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 85, Issue 1, 15 July 2011, Pages 197-205
Journal: Talanta - Volume 85, Issue 1, 15 July 2011, Pages 197-205
نویسندگان
Ciska Wyns, Lara Derycke, Bram Soenen, Selin Bolca, Dieter Deforce, Marc Bracke, Arne Heyerick,