کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739893 | 1046893 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oxidative stress and lipid mediators induced in alveolar macrophages by ultrafine particles
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کلمات کلیدی
Mkk1cPLA2BELLTB4PGE2DEPsPLA2AMSTXB25-LOESRiPLA2Ca2+-independent phospholipase A2COX5-Lipoxygenase - 5-لیپوکسیژناز8-Isoprostane - 8-ایزوپروستانp38 MAPK - P38 MAPKcyclooxygenase - آنزیم سیکلواکسیژنازphospholipase A2 - آنزیم فسفولیپاز A2 Secretory phospholipase A2 - اسید فسفولیپاز A2bromoenol lactone - بروموآنول لاکتونthromboxane B2 - ترومبوکسی B2Oxidative stress - تنش اکسیداتیوUltrafine particles - ذرات UltrafineDiesel exhaust particles - ذرات خروجی دیزلFree radicals - رادیکال آزادElectron spin resonance - رزونانس اسپین الکترونcytosolic phospholipase A2 - فسفولیپاز A2 سیتوزولLeukotriene B4 - لوکوترین B4Alveolar macrophages - ماکروفاژهای آلوئولارChemiluminescence - نورتابی شیمیایی یا کمی لومی نسانسLipid mediators - واسطه های لیپیدmitogen-activated protein kinase kinase 1 - پروتئین کربوکسی سدیم 1p38 mitogen-activated protein kinase - پروتئین کیناز متیوژن فعال p38Prostaglandin E2 - پروستاگلاندین E2elemental carbon - کربن عنصری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Oxidative stress and lipid mediators induced in alveolar macrophages by ultrafine particles Oxidative stress and lipid mediators induced in alveolar macrophages by ultrafine particles](/preview/png/10739893.png)
چکیده انگلیسی
In ambient aerosols, ultrafine particles (UFP) and their agglomerates are considered to be major factors contributing to adverse health effects. Reactivity of agglomerated UFP of elemental carbon (EC), Printex 90, Printex G, and diesel exhaust particles (DEP) was evaluated by the capacity of particles to oxidize methionine in a cell-free in vitro system for determination of their innate oxidative potential and by alveolar macrophages (AMs) to determine production of arachidonic acid (AA), including formation of prostaglandin E2 (PGE2), leukotriene B4 (LTB4), reactive oxygen species (ROS), and oxidative stress marker 8-isoprostane. EC exhibiting high oxidative potential induced generation of AA, PGE2, LTB4, and 8-isoprostane in canine and human AMs. Printex 90, Printex G, and DEP, showing low oxidative capacity, still induced formation of AA and PGE2, but not that of LTB4 or 8-isoprostane. Aging of EC lowered oxidative potential while still inducing production of AA and PGE2 but not that of LTB4 and 8-isoprostane. Cellular ROS production was stimulated by all particles independent of oxidative potential. Particle-induced formation of AA metabolites and ROS was dependent on mitogen-activated protein kinase kinase 1 activation of cytosolic phospholipase A2 (cPLA2) as shown by inhibitor studies. In conclusion, cPLA2, PGE2, and ROS formation was activated by all particle types, whereas LTB4 production and 8-isoprostane were strongly dependent on particles' oxidative potential. Physical and chemical parameters of particle surface correlated with oxidative potential and stimulation of AM PGE2 and 8-isoprostane production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 8, 15 April 2005, Pages 1080-1092
Journal: Free Radical Biology and Medicine - Volume 38, Issue 8, 15 April 2005, Pages 1080-1092
نویسندگان
Ingrid Beck-Speier, Niru Dayal, Erwin Karg, Konrad L. Maier, Gabriele Schumann, Holger Schulz, Manuela Semmler, Shinji Takenaka, Kurt Stettmaier, Wolf Bors, Andy Ghio, James M. Samet, Joachim Heyder,