کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755817 | 1050377 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of nuclear receptor NR5A2 increases Glut4 expression and glucose metabolism in muscle cells
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کلمات کلیدی
PGC1αdilauroyl phosphatidylcholineNR5A2PYGMDLPCHK2CYP8B1MyogeninMyoDGYS1FABP3CEPTacetyl-CoA carboxylase 2ACC2DMEMC2C12LPLT2DMMEF2CGLUT4PPARαCYP7A1Cpt1bS.D. - SD.VLDLR - VLDLstandard deviation - انحراف معیارmyogenic differentiation 1 - تمایز ماهیانه 1glucose transporter 4 - حمل کننده گلوکز 4Type 2 diabetes mellitus - دیابت نوع دوMuscle - عضلهLipoprotein lipase - لیپو پروتئین لیپازGlucose metabolism - متابولیسم گلوکزDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoMYOG - منوhexokinase 2 - هگزوکیناز 2cholesteryl ester transfer protein - پروتئین انتقال پروتئین کلسترول استPeroxisome proliferator activated receptor alpha - پروفیسیم پرولیفراتور فعال گیرنده آلفاcholesterol 7 alpha-hydroxylase - کلسترول 7 آلفا هیدروکسیلازVery low density lipoprotein receptor - گیرنده لیپوپروتئین بسیار کم چربی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
NR5A2 is a nuclear receptor which regulates the expression of genes involved in cholesterol metabolism, pluripotency maintenance and cell differentiation. It has been recently shown that DLPC, a NR5A2 ligand, prevents liver steatosis and improves insulin sensitivity in mouse models of insulin resistance, an effect that has been associated with changes in glucose and fatty acids metabolism in liver. Because skeletal muscle is a major tissue in clearing glucose from blood, we studied the effect of the activation of NR5A2 on muscle metabolism by using cultures of C2C12, a mouse-derived cell line widely used as a model of skeletal muscle. Treatment of C2C12 with DLPC resulted in increased levels of expression of GLUT4 and also of several genes related to glycolysis and glycogen metabolism. These changes were accompanied by an increased glucose uptake. In addition, the activation of NR5A2 produced a reduction in the oxidation of fatty acids, an effect which disappeared in low-glucose conditions. Our results suggest that NR5A2, mostly by enhancing glucose uptake, switches muscle cells into a state of glucose preference. The increased use of glucose by muscle might constitute another mechanism by which NR5A2 improves blood glucose levels and restores insulin sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 446, Issue 2, 4 April 2014, Pages 614-619
Journal: Biochemical and Biophysical Research Communications - Volume 446, Issue 2, 4 April 2014, Pages 614-619
نویسندگان
A. Bolado-Carrancio, J.A. Riancho, J. Sainz, J.C. RodrÃguez-Rey,