کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10803132 | 1055795 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of Bcl-2 and Bcl-xL anti-apoptotic protein expression by nuclear receptor PXR in primary cultures of human and rat hepatocytes
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کلمات کلیدی
PXRMRPBcl-xLPCNBcl-2MDR1GSTCyPOATPFBSDEXCYP3AUDPGTTNFCLO3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideMTT - MTTAntisense oligonucleotides - oligonucleotide antisensepregnenolone-16α-carbonitrile - pregnenolone-16α-کربناتریلUDP glucuronosyltransferase - UDP گلوکورونوسیل ترانسفرازstaurosporine - استوسوسورپینSpironolactone - اسپیرونولاکتونDexamethasone - دگزامتازونRifampicin - ریفامپینfoetal bovine serum - سرم جنین گاوCytochrome P450 - سیتوکروم پی۴۵۰tumor necrosis factor - فاکتور نکروز تومورPhenobarbital - فنوباربیتالMetyrapone - متریاپونMultidrug resistance-1 - مقاومت چند دارویی- 1multidrug resistance-associated protein - پروتئین مرتبط با مقاومت چند داروییorganic anion-transporting polypeptide - پلی اتیلن حمل و نقل آنیونی آلیclotrimazole - کلوتریمازولglutathione S-transferase - گلوتاتیون S-ترانسفرازPregnane X receptor - گیرنده پیش گران XCell - یاخته، سلولSCR - یکسوساز کنترلشده با سیلیکون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of Bcl-2 and Bcl-xL anti-apoptotic protein expression by nuclear receptor PXR in primary cultures of human and rat hepatocytes Regulation of Bcl-2 and Bcl-xL anti-apoptotic protein expression by nuclear receptor PXR in primary cultures of human and rat hepatocytes](/preview/png/10803132.png)
چکیده انگلیسی
The pregnane X receptor (PXR) plays a major role in the protection of the body by regulating the genes involved in the metabolism and elimination of potentially toxic xeno- and endobiotics. We previously described that PXR activator dexamethasone protects hepatocytes from spontaneous apoptosis. We hypothesise a PXR-dependent co-regulation process between detoxication and programmed cell death. Using primary cultured human and rat hepatocytes, we investigated to determine if PXR is implicated in the regulation of Bcl-2 and Bcl-xL, two crucial apoptosis inhibitors. In the present study we demonstrated that the treatment of primary cultured hepatocytes with PXR agonists increased hepatocyte viability and protects them from staurosporine-induced apoptosis. The anti-apoptotic capacity of PXR activation was correlated with Bcl-2 and Bcl-xL induction at both the transcriptional and protein levels in man and rats, respectively. The inhibition of PXR expression by antisense oligonucleotide abolished PXR activators Bcl-xL induction. Accordingly, PXR overexpression in HepG2 cells led to bcl-2 induction upon clotrimazole treatment and protects cells against Fas-induced apoptosis. Our results demonstrate that PXR expression is required for Bcl-2 and Bcl-xL up-regulation upon PXR activators treatment in human and rat hepatocytes. They also suggest that PXR may protect the liver against chemicals by simultaneously regulating detoxication and the apoptotic pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1745, Issue 1, 15 August 2005, Pages 48-58
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1745, Issue 1, 15 August 2005, Pages 48-58
نویسندگان
Nathalie Zucchini, Georges de Sousa, Béatrice Bailly-Maitre, Jean Gugenheim, Rémi Bars, Géraldine Lemaire, Roger Rahmani,