کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10815743 | 1058500 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Directional control of WAVE2 membrane targeting by EB1 and phosphatidylinositol 3,4,5-triphosphate
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FBSEB1IGF-IRPIP3HGFPAK1IGF-IF-actinPI 3-kinase - PI 3-کینازRNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNAStathmin - استاتمینیinsulin-like growth factor I - انسولین مانند عامل رشد IPH domain - دامنه PHActin filament - رشته آتیینfetal bovine serum - سرم جنین گاوHepatocyte growth factor - عامل رشد هپاتوسیتphosphatidylinositol 3,4,5-triphosphate - فسفاتیدیلینواستول 3،4،5-تری فسفاتPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازMicrotubules - میکروتوبول، ریزلوله هاCytoplasmic linker protein - پروتئین لینکر سیتوپلاسمیکClip - کلیپIGF-I receptor - گیرنده IGF-I
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Membrane targeting of WAVE2 along microtubules is mediated by a motor protein kinesin and requires Pak1, a downstream effector of Rac1. However, the mechanism by which WAVE2 targeting to the leading edge is directionally controlled remains largely unknown. Here we demonstrate that EB1, a microtubule plus-end-binding protein, constitutively associates with stathmin, a microtubule-destabilizing protein, in human breast cancer cells. Stimulation of the cells with insulin-like growth factor I (IGF-I) induced Pak1-dependent binding of the EB1-stathmin complex to microtubules that bear WAVE2 and colocalization of the complex with WAVE2 at the leading edge. Depletion of EB1 by small interfering RNA (siRNA) abrogated the IGF-I-induced WAVE2 targeting and stathmin binding to microtubules. On the other hand, chemotaxis chamber assays indicated that the IGF-I receptor (IGF-IR) was locally activated in the region facing toward IGF-I. In addition, IGF-I caused phosphatidylinositol 3-kinase (PI 3-kinase)-dependent production of phosphatidylinositol 3,4,5-triphosphate (PIP3) near activated IGF-IR and WAVE2 colocalization with it. Collectively, WAVE2-membrane targeting is directionally controlled by binding of the EB1-stathmin complex to WAVE2-bearing microtubules and by the interaction between WAVE2 and PIP3 produced near IGF-IR that is locally activated by IGF-I.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 22, Issue 3, March 2010, Pages 510-518
Journal: Cellular Signalling - Volume 22, Issue 3, March 2010, Pages 510-518
نویسندگان
Kazuhide Takahashi, Tacu Tanaka, Katsuo Suzuki,