کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10967086 | 1102804 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification by LC-MSE of outer membrane vesicle proteins of the Bexsero® vaccine
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کلمات کلیدی
MSEGNASBAOMVDOMVSerum bactericidal assay - آزمایش باکتری های سرمCoefficient of Variation - ضریب تغییرMass spectrometry - طیف سنجی جرمیOuter membrane vesicles - غشای بیرونی vesiclesNeisseria meningitidis - نایسریا مننژیتیس، مننگوکوکVaccine - واکسنQuantitative proteomics - پروتئومیک کمliquid chromatography - کروماتوگرافی مایع
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Meningococcal disease is a major cause of morbidity and mortality worldwide. Its epidemiology is currently dominated by five capsular serogroups (A, B, C, W, and Y). While effective vaccines already exist for serogroups A, C, W and Y, except for under clonal outbreaks, no vaccine was available against serogroup B. Recently, a four component vaccine, Bexsero®, designed to prevent infection caused by this serogroup, has been approved in Europe and other Countries for use in individuals from two months of age and older. The active components of this vaccine are three recombinant proteins identified by reverse vaccinology combined with detergent extracted outer membrane vesicles (DOMV) prepared from a New Zealand epidemic strain. Considering their intrinsic complexity, we performed additional characterization of DOMVs on top of the standard quality control testing carried out for batch release. We applied the Hi3 label-free LC-MSE methodology to qualitatively and quantitatively characterize the DOMV protein content. We first, successfully investigated the robustness and the accuracy of the methodology for the DOMV characterization and we then applied it to compare six DOMV production lots. Around 100 proteins were quantified from each preparation. When classified according to their predicted cellular localization, about 90% of the total protein amount belongs consistently to the outer membrane compartment. Using nonparametric hypothesis testing and complementary log-log linear regression, the quantifications of a subset of 21 proteins common to all lots and including approximately 90% (85-92%) of the total protein amount quantified in any DOMV lot were found consistent across lots. The relevance of these results is two-fold, showing that the Hi3 quantification methodology is robust for a broad range of proteins and indicating that the manufacturing process currently used for the production of the Bexsero® DOMV components is highly reproducible and consistent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 32, Issue 11, 5 March 2014, Pages 1273-1279
Journal: Vaccine - Volume 32, Issue 11, 5 March 2014, Pages 1273-1279
نویسندگان
Chiara Tani, Maria Stella, Danilo Donnarumma, Massimiliano Biagini, Pierino Parente, Alessandro Vadi, Claudia Magagnoli, Paolo Costantino, Fabio Rigat, Nathalie Norais,